γ-聚谷氨酸生物有机肥制备及其对土壤营养和青菜品质的影响OA

A bioorganic fertilizer containing γ-polyglutamic acid was prepared based on γ-polyglutamic acid-producing Bacillus subtilis A-5 to improve the utilization value of agricultural resources and develop sustainable agriculture.The preparation process comprises the following steps:(1)air drying and pulverizing the straw,and uniformly mixing the straw with livestock manure,soybean meal,and monosodium glutamate waste liquid to obtain a fermentation substrate,wherein the mass ratio of the livestock manure,straw,soybean meal,and monosodium glutamate waste liquid is 4～8:4～8:1～4:0～1;(2)inoculating Bacillus subtilis A-5 seed inoculum into the fermentation substrate,uniformly and fermenting to obtain the γ-polyglutamic acid bioorganic fertilizer.The field plot experiment was used to study its growth promotion effect on Brassica chinensis L.and its application in reducing high temperature damage of crop.The results show that the γ-polyglutamic acid bioorganic fertilizer in this study,which uses agricultural waste as fermentation substrate,is cost-effective and can promote crop nutrient uptake,increase yield and quality,improve soil physicochemical properties,and improve crop resistance to high-temperature weather.