肉类研究2023，Vol.37Issue(12)：7-15,9.DOI:10.7506/rlyj1001-8123-20231115-102
功能性鱼软骨多糖制备工艺优化及食用安全性评价
Optimization of the Preparation Process of Functional Fish Cartilage Polysaccharide and Its Safety Evaluation for Consumption
摘要
Abstract
Low-molecular-mass chondroitin sulfate(CS)have attracted wide attention due to their better bioavailability and biological activity than highmolecular-mass CS.To obtain low-molecular-mass sulfate chondropolysaccharides with higher anti-colorectal cancer activity,sturgeon chondroitin sulfate(SCS)from the hybrid sturgeon Acipenser schrenckii×Huso dauricus was enzymatically depolymerized.Response surface methodology was used to optimize the enzymatic process based on inhibitory activity against the proliferation of HT-29 cells.The optimal enzymatic conditions were determined as 0.103 IU/mg,1.005 mg/mL and 87 min for enzyme dosage,substrate concentration,and enzymatic digestion time,respectively.Furthermore,SCS-F2 with low molecular mass and high anticancer activity was prepared by ultrafiltration.In order to explore its safety for consumption,the acute toxicity of SCS-F2 was evaluated by using an animal model.The experimental results showed that SCS-F2 has no adverse effects on body mass,food intake,blood biochemical indexes or visceral tissues while reducing the number of aberrant colonic crypts,indicating that SCS-F2 has inhibitory and preventive effects on colorectal cancer and meets the safety requirements of food and health products.Therefore,SCS-F2 is safe and non-toxic,and has anti-colorectal cancer activity.关键词
低分子质量硫酸软骨多糖/制备/结直肠癌/安全性Key words
low-molecular-mass chondroitin sulfate/preparation/colorectal cancer/safety分类
轻工纺织引用本文复制引用
武瑞赟,桂萌,刘子宇,Tharushi S. Shinali,尚楠..功能性鱼软骨多糖制备工艺优化及食用安全性评价[J].肉类研究,2023,37(12):7-15,9.基金项目
现代农业产业技术体系北京市渔业创新团队项目(BCIC07-2023-13) （BCIC07-2023-13）
