水稻维生素B1合成相关基因OsTHIC的功能研究OA北大核心CSTPCD

[Objective]Vitamin B1(VB1)is a biologically essential micronutrient that acts as a cofactor for several enzymes and is involved in important cellular metabolic pathways.However,the synthesis pathway of vitamin B1 in rice remains elusive.The aim of this study is to elucidate the biological functions of OsTHIC,a gene related to vitamin B1 synthesis in rice.[Method]We used a combination of mutagenesis techniques,pigment measurement,Mutmap+,gene editing,and non-targeted metabolomics analysis to clone the target gene and analyze its biological functions.[Result]An albino lethal mutant,wll1(white leaf and lethal 1),was obtained from EMS-induced mutant pool of japonica rice variety Nipponbare.The fourth leaf of wll1 had albino phenotype and the albino phenotype spread to other leaves,ultimately leading to seedling death.The photosynthetic chlorophyll and carotenoid contents of the wll1 mutant was significantly lower than that of the wild type(WT).The target gene OsTHIC involved in VB1 synthesis was determined by MutMap+ and gene editing technology.OsTHIC gene was expressed in various tissues of rice,with the highest expression in the leaves and OsTHIC protein was localized in chloroplasts.The VB1 content in the wll1 was significantly lower than that of the WT.Exogenous VB1 restored the mutated phenotype of wll1.Exogenous VB1 and OsTHIC mutation both affected the expressions of genes related to VB1 synthesis.Non-targeted metabolome sequencing analysis showed that differential metabolites between wild type and wll1 were significantly enriched in amino acid biosynthesis,cofactor biosynthesis,ABC transporters,and aminoacyl-tRNA biosynthesis pathways.[Conclusion]OsTHIC plays an important role in the growth and development of rice by regulating the content of VB1 and participating in metabolic processes such as amino acid synthesis.