生物技术通报2024，Vol.40Issue(2)：120-129,10.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0697
燕麦全基因组SSR位点鉴定及多态性标记开发
Identification of SSR Loci and Development of Polymorphic Markers in Whole Genome of Oat
摘要
Abstract
[Objective]Due to the limited number of SSR markers developed in oat,it is difficult to meet the needs of functional gene mapping and polymorphism marker development in oat.[Method]Using the published reference genome,the SSR loci of the whole oat genome were retrieved by TBtools software,and their distribution characteristics were analyzed by Excel and SPSS software.[Result]The SSR was abundant and varied in oat genome.A total of 828138 SSR loci were identified in the whole genome,with an average density of 78.16 SSR/Mb and an average distance of 12.90 kb.Single,double and trinucleotide repeat types were dominant.The repetitions of the primitives were mainly 5 and 6,and the A/T,AG/CT and AAC/GTT repetitions were the dominant primitives.The SSR loci of oat genome showed moderate polymorphism,with length ranging from 11 to 1587 bp.Through the systematic identification and analysis of the whole oat gene SSR locus,52 pairs of polymorphic primers were developed and validated in the test material.[Conclusion]The development of SSR primers in the reference genome of"Sanfensan"naked oat is effective and feasible,and can be used for further experimental research.关键词
燕麦/皮裸/SSR标记/引物开发/多态性Key words
oats/skin/SSR markers/primer development/polymorphism引用本文复制引用
陈凯凌,武涛,徐逸群,高佳,张美俊,李欣,贾举庆..燕麦全基因组SSR位点鉴定及多态性标记开发[J].生物技术通报,2024,40(2):120-129,10.基金项目
杂粮种质资源创新与分子育种国家实验室(筹)自主研发项目(202204010910001-26),山西省回国留学人员科研资助项目(2020-067) （筹）
