陆地棉HD-Zip家族全基因组鉴定及响应非生物胁迫的表达分析OA北大核心CSTPCD

[Objective]HD-Zip(Homeodomain and Leucine zipper)family is one of the plant-specific transcription factor families,which plays an important role in plant growth and development and stress response.In this study,the members of GhHDZ gene family were identified from the whole genome of Gossypium hirsutum L.and the expression characteristics of related genes were analyzed to provide support for further research.[Method]The members of GhHDZ gene family were identified from TM-1 genome of G.hirsutum L.by bioinformatics,and their physicochemical properties,phylogenetic relationship,chromosome location,gene replication,gene structure and cis-acting elements in promoter region were analyzed.Transcriptome data and real-time quantitative polymerase chain reaction(RT-qPCR)were used to analyze the expression patterns of GhHDZ family genes under different abiotic stresses.The subcellular localization of the target protein was detected by tobacco transient transformation method,and the function of the target gene was verified by transgenic overexpression method.[Result]A total of 205 GhHDZ genes were identified in upland cotton genome,and the GhHDZ family was divided into 4 subgroups by phylogenetic analysis.Fragment replication was the main reason for the evolution of the GhHDZ gene family,and the gene family has undergone a strong purification selection.GhHDZ family members had potential diversity of biological functions.The analysis of abiotic stress transcriptome combined with RT-qPCR analysis of 10 GhHDZ genes showed that GhHDZ12,GhHDZ46 and GhHDZ119 positively responded to cold stress,GhHDZ15 and GhHDZ188 negatively responded to cold stress,GhHDZ15,GhHDZ46,GhHDZ50,GhHDZ76,GhHDZ116,GhHDZ146,GhHDZ176,GhHDZ188 positively responded to heat stress,and GhHDZ15,GhHDZ50,GhHDZ76,GhHDZ116,GhHDZ119,GhHDZ146,GhHDZ176,GhHDZ188 positively responded to salt stress.GhHDZ12,GhHDZ15,GhHDZ50,GhHDZ116,GhHDZ119,GhHDZ176,GhHDZ188 positively responded to drought stress,while GhHDZ76 negatively responded to drought stress,and it was found that the response times of these genes to various stresses were different.Further study on the function of GhHDZ146 showed that the gene was localized in the nucleus and heterologous overexpression in Arabidopsis,which significantly enhanced the tolerance to salt stress.[Conclusion]205 members of GhHDZ gene family are systematically identified from G.hirsutum L.within the whole genome.Different genes have different responses to various stresses,and there are differences in expression patterns.The positive response function of GhHDZ146 to salt stress is confirmed.