生物技术通报2024，Vol.40Issue(2)：130-145,16.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0869
陆地棉HD-Zip家族全基因组鉴定及响应非生物胁迫的表达分析
Genome-wide Identification of HD-Zip Gene Family in Gossypium hirsutum L.and Expression Analysis in Response to Abiotic Stress
摘要
Abstract
[Objective]HD-Zip(Homeodomain and Leucine zipper)family is one of the plant-specific transcription factor families,which plays an important role in plant growth and development and stress response.In this study,the members of GhHDZ gene family were identified from the whole genome of Gossypium hirsutum L.and the expression characteristics of related genes were analyzed to provide support for further research.[Method]The members of GhHDZ gene family were identified from TM-1 genome of G.hirsutum L.by bioinformatics,and their physicochemical properties,phylogenetic relationship,chromosome location,gene replication,gene structure and cis-acting elements in promoter region were analyzed.Transcriptome data and real-time quantitative polymerase chain reaction(RT-qPCR)were used to analyze the expression patterns of GhHDZ family genes under different abiotic stresses.The subcellular localization of the target protein was detected by tobacco transient transformation method,and the function of the target gene was verified by transgenic overexpression method.[Result]A total of 205 GhHDZ genes were identified in upland cotton genome,and the GhHDZ family was divided into 4 subgroups by phylogenetic analysis.Fragment replication was the main reason for the evolution of the GhHDZ gene family,and the gene family has undergone a strong purification selection.GhHDZ family members had potential diversity of biological functions.The analysis of abiotic stress transcriptome combined with RT-qPCR analysis of 10 GhHDZ genes showed that GhHDZ12,GhHDZ46 and GhHDZ119 positively responded to cold stress,GhHDZ15 and GhHDZ188 negatively responded to cold stress,GhHDZ15,GhHDZ46,GhHDZ50,GhHDZ76,GhHDZ116,GhHDZ146,GhHDZ176,GhHDZ188 positively responded to heat stress,and GhHDZ15,GhHDZ50,GhHDZ76,GhHDZ116,GhHDZ119,GhHDZ146,GhHDZ176,GhHDZ188 positively responded to salt stress.GhHDZ12,GhHDZ15,GhHDZ50,GhHDZ116,GhHDZ119,GhHDZ176,GhHDZ188 positively responded to drought stress,while GhHDZ76 negatively responded to drought stress,and it was found that the response times of these genes to various stresses were different.Further study on the function of GhHDZ146 showed that the gene was localized in the nucleus and heterologous overexpression in Arabidopsis,which significantly enhanced the tolerance to salt stress.[Conclusion]205 members of GhHDZ gene family are systematically identified from G.hirsutum L.within the whole genome.Different genes have different responses to various stresses,and there are differences in expression patterns.The positive response function of GhHDZ146 to salt stress is confirmed.关键词
陆地棉/GhHDZ基因家族/系统进化/非生物胁迫/表达模式Key words
Gossypium hirsutum L./GhHDZ gene family/phylogeny/abiotic stress/expression pattern引用本文复制引用
吴翠翠,肖水平..陆地棉HD-Zip家族全基因组鉴定及响应非生物胁迫的表达分析[J].生物技术通报,2024,40(2):130-145,16.基金项目
山西省自然科学研究面上项目(202103021224133),山西农业大学"引进人才科研启动工程"项目(2023BQ23),江西省自然科学基金(20224BAB205023),江西省现代农业产业技术体系建设专项资金(JXARS-22),棉花生物育种与综合利用全国重点实验室开放课题基金(CB2023A06),中央引导地方科技发展资金项目(北疆棉花优良种质重要性状的优异位点评价及利用) （202103021224133）
