细胞糖酵解对猪流行性腹泻病毒复制的影响OACSTPCD

This study was to investigate the changes in glucose metabolism in IPEC-J2 cells infected with porcine epidemic diarrhea virus(PEDV)and the effect of cell glycolysis on PEDV replication.The level of glycolysis related enzyme Hexokinase 2(HK2)in IPEC-J2 cells at 36 h post PEDV infection was detected by quantitative PCR and Western blot.Then,the effect of cell glycolysis on PEDV replication was further identified by adding glycolysis inhibitor 2-Deoxy-D-glucose(2-DG).Finally,the replication of PEDV under the conditions of nor-mal concentration of glucose(5.5 mmol/L)and high concentration of glucose(11.1 mmol/L and 22.2 mmol/L)conditions was detected to investigate the effect of glucose concentration on PEDV replication.The results showed that PEDV infection significantly increased the expres-sion of the glycolysis rate limiting enzyme HK2,which means that PEDV infection could enhance the glycolysis in IPEC-J2 cells.The en-hanced level of cell glycolysis was conducive to the replication of PEDV.Increasing glucose concentration within a certain range could pro-mote the replication of PEDV.This research provided a theoretical basis for preliminary elucidation of the infection mechanism of PEDV and comprehensive prevention and control of coronaviruses.