调脾承气汤改善食积小鼠胃部消化功能的转录组学研究OA北大核心CSTPCD
Transcriptomic analysis of the molecular mechanism of Tiaopi Chengqi decoction improving gastric digestive function in mice with food accumulation
目的:基于转录组学探索调脾承气汤改善高热量和高蛋白食物导致积食小鼠的分子机制.方法:C57小鼠被随机分为正常对照组、模型组、调脾承气汤低剂量组(145 mg/m L)、调脾承气汤高剂量组(580 mg/mL)、多潘立酮组(0.15 mg/mL).对实验小鼠进行一般状态观察和平均食量统计,测定各组小鼠胃排空及肠推进率.H&E染色观察胃组织病理变化;PAS染色观察胃组织糖元变化;比色法测定胃蛋白酶活力;酸碱滴定法测胃内容物的pH值.转录组测序分析胃组织差异基因,并对差异基因制作火山图和聚类热图,KEGG对差异基因进行信号通路富集分析.RT-qPCR验证筛选得到的差异基因.结果:积食小鼠在调脾承气汤治疗后小鼠体质量和平均食量增加(P＜0.05),积食小鼠肠推进率和胃排空速度加快(P＜0.05).调脾承气汤能够保护胃组织结构和胃组织糖元降解,提升胃蛋白酶活力(P＜0.05)和降低胃内容物pH(P＜0.05).转录组结果显示调脾承气汤可以调节Acox2和cilp2的表达,调节脂肪消化吸收、蛋白质消化吸收、胰腺分泌信号.RT-qPCR显示,相较于模型组,调脾承气汤上调Acox2(P＜0.05),下调cilp2(P＜0.05)的mRNA水平.结论:调脾承气汤可以改善高热量和高蛋白食物导致积食小鼠的消化功能障碍,其机制涉及到调节脂肪和糖类代谢基因Acox2和cilp2,以及胰腺分泌信号.
AIM:To explore the molecular mecha-nism of Tiaopi Chengqi decoction(TpCqD)improv-ing hyperthermia and high-protein food-induced hy-perphagia mice based on transcriptomics.METH-ODS:C57 mice were randomly divided into a con-trol group,model group,low-dose TpCqD group,high-dose TpCqD group,and domperidone group.The general condition of the experimental mice was observed and the average food intake was counted,and the rate of gastric emptying and intes-tinal propulsion was determined for each group of mice.H&E staining was used to observe pathologi-cal changes in gastric tissue.PAS staining was used to observe glycogen changes in gastric tissue.Pep-sin activity was determined by colorimetry.pH val-ue of gastric contents was measured by acid-base titration.Transcriptome sequencing was used to an-alyze the differential genes in gastric tissue,a volca-no map and a cluster heat map were made for the differential genes,and KEGG was used to analyze the signal pathway enrichment of the differential genes.RT-qPCR verified the differential genes ob-tained by screening.RESULTS:After treatment with TpCqD,the body weight and average food intake of mice with food accumulation increased(P＜0.05),and the intestinal propulsion rate and gastric emp-tying speed of mice with food accumulation accel-erated(P＜0.05).TpCqD could protect gastric tissue structure and glycogen degradation,increase pep-sin activity(P＜0.05),and reduce gastric content pH(P＜0.05).Transcriptome results showed that TpCqD could regulate the expression of Acox2 and cilp2,regulate fat digestion and absorption,protein diges-tion and absorption,and pancreatic secretion sig-nals.RT-qPCR showed that compare with model group,TpCqD up-regulated Acox2(P＜0.05)and down-regulated the mRNA level of cilp2(P＜0.05).CONCLUSION:TpCqD ameliorated digestive dys-function in mice with high-calorie and high-protein diets leading to food accumulation involving the regulation of the fat and sugar metabolism genes Acox2 and cilp2,and pancreatic secretory signaling.
王小芸;赵怀舟;仝立国;吉海杰;杨钤;王平;卢海燕;宋明锁
山西省中医药研究院,山西省中医院,中医基础理论研究所山西省中医药研究院,山西省中医院,中心实验室山西中医药大学基础医学院山西省中医药研究院,山西省中医院,儿科,太原 030012,山西
药学
调脾承气汤转录组消化不良胃肠功能
Tiaopi Chengqi decoctiontranscrip-tomedyspepsiagastrointestinal function
《中国临床药理学与治疗学》 2024 (003)
252-259 / 8
山西省中医药管理局资助项目(2023ZYYC2005);山西省中医药研究院基金资助项目(202310)
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