宿主蛋白BST-2抑制非洲猪瘟病毒体外复制的研究OA北大核心CSTPCD

African swine fever(ASF)is a highly lethal infectious disease caused by the African swine fever virus(ASFV).Bone marrow stromal cell antigen 2(BST-2,also known as Tetherin/HM1.24/CD317),which binding virus particles to the cell surface,internalizing them,and subsequently degrading them in lysosomes,has been identified as a novel host factor that restricts the release of enveloped virus particles.That's why BST-2 can effectively inhibits virus replication.Moreover,BST-2 plays a crucial role in the host's antiviral effect.Notably,previous research conducted in our laboratory has demon-strated that pregnant pig serum can enhance the replication of ASFV.Present study utilized ITRAQ to analyze the disparity in proteins between empty pregnant pig serum and pregnant pig serum,revealing that BST-2 protein was one of the top ten down-regulated proteins.In order to investigate the relation of host protein BST-2 and ASFV,the effect of ASFV infection on BST-2 expression was assessed through Western-blot and real-time fluorescence quantitative PCR(RT-qPCR).Furthermore,we constructed and synthesized the eukaryotic expression plasmid of BST-2 and siRNA interference sequence.Using RT-qPCR and Western-blot analysised the regulation function of exogenous overexpression and knock-down of BST-2 on the in vitro replication of ASFV.The findings revealed a significant inhibition of ASFV repli-cation upon exogenous overexpression of BST-2,while knock-down of BST-2 facilitated ASFV replication.This article demonstrated the overexpression and knockdown of BST-2,establishing its role as a host antiviral molecule that effectively impedes the replication of ASFV in vitro.These findings offer a novel avenue for future investigations into the antiviral function of the host proteins against ASFV,while also providing a new idea for the prevention and control of ASF.