中国兽医科学2024，Vol.54Issue(2)：143-150,8.DOI:10.16656/j.issn.1673-4696.2024.0031
宿主蛋白BST-2抑制非洲猪瘟病毒体外复制的研究
Study on host protein BST-2 inhibiting ASFV's replication in vitro
摘要
Abstract
African swine fever(ASF)is a highly lethal infectious disease caused by the African swine fever virus(ASFV).Bone marrow stromal cell antigen 2(BST-2,also known as Tetherin/HM1.24/CD317),which binding virus particles to the cell surface,internalizing them,and subsequently degrading them in lysosomes,has been identified as a novel host factor that restricts the release of enveloped virus particles.That's why BST-2 can effectively inhibits virus replication.Moreover,BST-2 plays a crucial role in the host's antiviral effect.Notably,previous research conducted in our laboratory has demon-strated that pregnant pig serum can enhance the replication of ASFV.Present study utilized ITRAQ to analyze the disparity in proteins between empty pregnant pig serum and pregnant pig serum,revealing that BST-2 protein was one of the top ten down-regulated proteins.In order to investigate the relation of host protein BST-2 and ASFV,the effect of ASFV infection on BST-2 expression was assessed through Western-blot and real-time fluorescence quantitative PCR(RT-qPCR).Furthermore,we constructed and synthesized the eukaryotic expression plasmid of BST-2 and siRNA interference sequence.Using RT-qPCR and Western-blot analysised the regulation function of exogenous overexpression and knock-down of BST-2 on the in vitro replication of ASFV.The findings revealed a significant inhibition of ASFV repli-cation upon exogenous overexpression of BST-2,while knock-down of BST-2 facilitated ASFV replication.This article demonstrated the overexpression and knockdown of BST-2,establishing its role as a host antiviral molecule that effectively impedes the replication of ASFV in vitro.These findings offer a novel avenue for future investigations into the antiviral function of the host proteins against ASFV,while also providing a new idea for the prevention and control of ASF.关键词
非洲猪瘟病毒/BST-2/天然免疫/宿主蛋白/抑制/体外复制Key words
Africanswinefevervirus/BST-2/innateimmunity/hostprotein/inhibit/in vitro replication分类
农业科技引用本文复制引用
赵美玉,赵思越,李平,郑海学,栗孟飞,张克山,杨行,史喜绢,张大俊,赵登率,陈玲玲,别鑫恬,闫文倩,陈国辉..宿主蛋白BST-2抑制非洲猪瘟病毒体外复制的研究[J].中国兽医科学,2024,54(2):143-150,8.基金项目
国家重点研发计划项目(2021YFD1801300) （2021YFD1801300）
国家生猪产业体系(CARS-35) （CARS-35）
中央高校基本科研业务费专项 （）
国家生猪技术创新中心先导科技项目(NCTIP-XD/C03) （NCTIP-XD/C03）
甘肃省科技重大专项(22ZD6NA001) （22ZD6NA001）
"十四五"广东省农业科技创新十大主攻方向"揭榜挂帅"项目(2022SDZG02) （2022SDZG02）
中国农业科学院科技创新工程(CAAS-ASTIP-2022-LVRI) （CAAS-ASTIP-2022-LVRI）
中国农业科学院基础科学中心重大任务(CAAS-CSLPDC-202302) （CAAS-CSLPDC-202302）
