中国食品学报2024，Vol.24Issue(2)：303-314,12.DOI:10.16429/j.1009-7848.2024.02.029
氮掺杂碳量子点荧光法检测猪肉中的四环素
Fluorescent Determination of Tetracycline in Pork Based on Nitrogen-doped Carbon Quantum Dots
摘要
Abstract
As a common antibiotic,tetracycline(TC)is extensively used in the prevention and treatment bacterial dis-eases in animals and is also used as a feed additive to promote the growth of animals.In this paper,a fluorescence quenching method based on nitrogen doped carbon quantum dots(N-CQDs)derived from pineapple peel was established for the rapid detection of TC.The blue fluorescent N-CQDs with high quantum yield were prepared by the hydrothermal method using pineapple peel and diethylenetriamine as precursors.The structure of N-CQDs was characterized by trans-mission electron microscope,fourier infrared spectroscopy and X-ray photoelectron spectroscopy.The results showed that the average particle size of N-CQDs was about 3.53 nm,which was mainly composed of C,N and O,and the surface has oxygen-containing and nitrogen-containing groups.The fluorescence of N-CQDs was effectively quenched by static quenching and internal filtering effect.Under the optimal experimental conditions,the method had good linearity within 0.3-40 μg/mL,and the detection limit was calculated to be 89.26 μg/L.Moreover,the detection method has been applied to the determination of TC in pork,the recovery rate was 98.40％-106.02％.The results showed that the detection method could be applied to the rapid determination of TC in pork.关键词
菠萝皮/氮掺杂碳量子点/荧光检测/四环素Key words
pineapple peel/nitrogen doped carbon quantum dots/fluorescent detection/tetracycline引用本文复制引用
周彤,毋晨,赵文,李明,彭汝艳,周茜..氮掺杂碳量子点荧光法检测猪肉中的四环素[J].中国食品学报,2024,24(2):303-314,12.基金项目
河北省现代农业产业技术体系生猪创新团队项目(HBCT2018110205) （HBCT2018110205）
