植物研究2024，Vol.44Issue(2)：232-238,7.DOI:10.7525/j.issn.1673-5102.2024.02.008
一株拟南芥宽叶形突变体atscamp的分离鉴定
Isolation and Identification of a Novel Enlarged Leaf Mutant atscamp in Arabidopsis thaliana
摘要
Abstract
Leaf is the main photosynthetic organ,and the leaf morphology beneficial for photosynthesis has become an important breeding target.atscamp was screened from the Arabidopsis thaliana mutant library(about 6 000 lines)with wide leaves.Tail-PCR analysis revealed a T-DNA insertion at the AT1G11180 locus,which encodes a secretory carrier membrane protein(SCAMP).RT-PCR showed that the transcriptional expression level of the gene was basically zero.The leaf width and area of the mutant were significantly larger than those of wild type plants(P＜0.01),while the crown diameter was essentially unchanged.Additionally,chlorophyll content,chlorophyll maximium fluorescence,and photosystem Ⅱ potential photochemical efficiency were all increased in the atscamp mutant(P＜0.05).Correspondingly,the mutants showed significant increases in transpiration coefficient(Tr),net photosynthetic rate(Pn),and leaf water use efficiency(WUE)(P＜0.05).Spatial-temporal specific expression analysis of the AT1G11180 gene revealed that the gene was highly expressed only in leaves,with low expression levels in other organs,and the gene expression gradually increased with the development and maturity of plants.The results suggested that AtSCAMP might play an important role in leaf shape development.关键词
拟南芥/AtSCAMP基因/叶形/突变体Key words
Arabidopsis thaliana/AtSCAMP gene/leaf shape/mutant分类
生物科学引用本文复制引用
郝雪峰,贾晓宇,曹海艳,亢春霞,裴雁曦..一株拟南芥宽叶形突变体atscamp的分离鉴定[J].植物研究,2024,44(2):232-238,7.基金项目
山西省应用基础研究计划项目(20210302123091) （20210302123091）
太原师范学院研究生教育创新项目(SYYJSYC-2320). （SYYJSYC-2320）
