轻工学报2024，Vol.39Issue(1)：1-11,11.DOI:10.12187/2024.01.001
结构脂质的酶法合成及应用酶工程提高其合成效果的研究进展
Research progress in enzymatic synthesis of structured lipids and improvement of the synthetic effect by enzyme engineering
摘要
Abstract
The utilization of lipase in synthesis structured lipids(SLs)from natural oils confers additional health properties and effectively enhances their value-added potential.This paper provided a comprehensive review of recent studies on the enzymatic preparation of SLs,summarizing the advancements in structural design and immo-bilized enzyme engineering technology to improve the efficiency of their synthesis by enzymatic approaches.The sn-1,3 regiospecific lipase enabled the precise synthesis of SLs,while employing stepwise variable temperature reactions,supercritical fluid reactions,vacuum reactions,and ultrasonic-assisted methods could inhibit the acyl migration effects and further enhance product yield.Furthermore,structural design could enhance the catalytic efficiency,stability,and substrate specificity of lipases to elevate the efficiency and yield in enzymatic synthesis of SLs.Immobilized enzyme technology could enhance enzyme reactivity and stability while reducing the costs of enzymatic synthesis of SLs.In the future,the high-performance enzymes should be developed by combining structural modifi-cation and novel immobilization technology on the basis of the clearer understanding of their structure-function char-acteristics.These insights would contribute to innovation and advancement in efficient and precise enzymatic syn-thesis processes for SLs.关键词
结构脂质/脂肪酶/酶法合成/酶工程/结构设计/固定化Key words
structured lipids/lipase/enzymatic synthesis/enzyme engineering/structure design/immobilization分类
轻工纺织引用本文复制引用
赵泽鑫,周灵,韩美玲,许源,蔡俊..结构脂质的酶法合成及应用酶工程提高其合成效果的研究进展[J].轻工学报,2024,39(1):1-11,11.基金项目
国家自然科学基金青年基金项目(32302019) （32302019）
湖北省教育厅科技攻关计划青年人才项目(Q20266407) （Q20266407）
