昆明医科大学学报2024，Vol.45Issue(2)：7-13,7.DOI:10.12259/j.issn.2095-610X.S20240202
HK2和VDAC1在二乙酰吗啡致心肌细胞凋亡中的作用
Role of HK2 and VDAC1 in Diacetylmorphine-induced Cardiomyocyte Apoptosis
摘要
Abstract
Objective To investigate the role of HK2 and VDAC1 in diacetylmorphine-induced cardiomyocyte apoptosis.Methods A dose-escalation method was used to establish a rat model of diacetylmorphine addiction.Forty SD rats were randomly divided into three groups,the normal group(n=10)was injected with an equal amount of saline subcutaneously,the model group(n=15)was injected with 5 mg/kg of diacetylmorphine for the first time,and then the dose was increased by 2.5 mg/(kg·d)day by day for 20 days,and the group of model +10 D(n=15)continued to increase the dose based on the model group up to the 10th day.Lactate dehydrogenase(LDH)and glutamic oxaloacetic transaminase(GOT)were detected by ELISA;HE staining was used to observe the pathological changes of myocardial tissues in each group;TUNEL staining was used to detect apoptosis in myocardial tissues in each group;and immunohistochemistry,RT-q-analysis,and immunochemistry were used to detect apoptosis in myocardial tissues in each group.Immunohistochemistry,RT-qPCR and Western bl-ot were used to detect the mRNA and protein expression of HK2,VDAC1 and apoptosis-related factors.Results HE staining revealed that myocardial tissues exhibited different degrees of damage with the prolongation of diacetylmorphine intervention.Compared with the normal group,serum LDH,GOT content and myocardial apoptosis rate increased in the model group,mRNA and protein levels of HK2 and anti-apoptotic factor Bcl-2 decreased,mRNA and protein levels of VDAC1 and pro-apoptotic factors Bax and Caspase-3 increased,and the protein level of Clevead Caspase-3 increased;in the model +10 D group the above indexes,there was a statistically significant difference(P<0.05).Conclusion Diacetylmorphine can cause cardiomyocyte apoptosis,and VDAC1 may be involved in the process of cardiomyocyte apoptosis caused by diacetylmorphine.关键词
二乙酰吗啡/心肌凋亡/己糖激酶2/电压依赖性阴离子通道1/裂解半胱氨酸天冬氨酸蛋白酶-3Key words
Diacetylmorphine/Cardiac apoptosis/HK2/VDAC1/Clevead Caspase-3分类
医药卫生引用本文复制引用
肖锦玲,管雅玲,朱森森,庄梦婕,苏丽萍,蒲红伟..HK2和VDAC1在二乙酰吗啡致心肌细胞凋亡中的作用[J].昆明医科大学学报,2024,45(2):7-13,7.基金项目
国家自然科学基金资助项目(8216020119) （8216020119）
新疆维吾尔自治区研究生科研创新项目(XJ2023G192) （XJ2023G192）
