KRAS基因3'UTR多态性与云南汉族人群宫颈癌及宫颈上皮内瘤变的相关性OACSTPCD
Correlation of KRAS Gene 3'UTR Polymorphisms with Cervical Cancer and Cervical Intraepithelial Neoplasia in Chinese Han Population in Yunnan Province
目的 探究KRAS基因中 3'UTR区域的rs712 和rs7973450 位点与云南汉族人群宫颈癌(cervical cancer,CC)和宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)的相关性.方法 共纳入CIN患者 461 例、CC患者 961 例及其健康对照 983 例,采用TaqMan探针法进行基因分型检测,并分析 2 个SNP位点与CIN及CC的相关性.结果 rs7973450 位点的A等位基因可能是CIN(P = 0.004,OR = 0.651,95%CI 0.487～0.871)与CC(P = 7.00×10-4,OR = 0.667,95%CI 0.529～0.844)发生的保护性因素.rs712 位点在CIN组、CC组和对照组间等位基因和基因型分布频率的差异无统计学意义(P>0.017);单倍型分析的结果显示,单倍型rs712A-rs7973450G与更高的 CIN(P = 4.00×10-4,OR = 1.714,95%CI 1.269～2.314)和 CC(P = 3.84×10-5,OR = 1.667,95%CI 1.305～2.131)发生风险相关;单倍型rs712A-rs7973450A则与更低的CC发生风险相关(P = 0.012,OR = 0.790,95%CI 0.658～0.950).结论 位于KRAS基因 3'UTR区域的SNP位点rs7973450 的A等位基因可能是云南汉族人群CIN和CC发生的保护性因素.
Objective To investigate the correlation between rs712 and rs7973450 located at the 3'UTR region of the KRAS gene and the risk of cervical cancer(CC)and cervical intraepithelial neoplasia(CIN)in Chinese Han population in Yunnan province.Methods A total of 2405 individuals(461 subjects with CIN,961 subjects with CC and 983 healthy controls)were enrolled.The SNPs were genotyped used TaqMan assay and the correlation of these SNPs with CIN and CC was analyzed.Results The A allele of rs7973450 might be a protective factor for the occurrence of CIN(P = 0.004,OR= 0.651,95%CI 0.487～0.871)and CC(P = 7.00×10-4,OR= 0.667,95%CI 0.529～0.844).There was no significant difference in allelic and genotypic distribution of rs712 among CIN,CC and Control groups(P>0.017).The haplotype assay showed thatrs712A-rs7973450G was associated with increased risk of CIN(P = 4.00×10-4;OR= 1.714,95%CI 1.269～2.314)and CC(P = 3.84×10-5,OR= 1.667,95%CI 1.305～2.131).While haplotype rs712A-rs7973450A was associated with a lower risk of CC(P = 0.012,OR= 0.790,95%CI 0.658～0.950).Conclusion The A allele of rs7973450 in 3'UTR of KRAS gene might be the protective factor for the occurrence of CIN and CC in a Chinese Han population in Yunnan province.
郭妮;张承;洪超;刘伟鹏;姚宇峰;严志凌
中国医学科学院 & 北京协和医学院医学生物学研究所,云南 昆明 650118镇雄县人民医院妇科,云南 镇雄 657200镇雄县人民医院妇科,云南 镇雄 657200||昆明医科大学第三附属医院妇科,云南 昆明 650118
临床医学
KRAS基因单核苷酸多态性宫颈癌宫颈上皮内瘤变云南汉族人群相关性
KRASSingle nucleotide polymorphismCervical cancerCervical intraepithelial neoplasiaHan population in Yunnan
《昆明医科大学学报》 2024 (002)
14-22 / 9
国家自然科学基金资助项目(82103190);云南省基础研究计划项目(202201AY070001-139;202201AU070163)
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