白藜芦醇对后肢去负荷雄性大鼠生殖损伤的对抗作用OA北大核心CSTPCD

To research the antagonistic effect of resveratrol on reproductive injury in hind-limp un-loading male rats.The 30 experimental rats were randomly divided into placebo control group,hind-limp unloading group,and HU+Res group.The HU+Res group was given resveratrol at the dose of 30 mg·kg-1 respectively every two days,and the placebo control group and hind-limp unloading groups were given equal volume of physiological saline.After 28 days,all rats were executed,and testes and epi-didymis were taken for histopathology detection,tissue biochemical index detection,and protein expres-sion analysis of testicular tissue.Compared with the placebo control group,the weight of testes and epi-didymis in the hind-limp unloading group was significantly reduced,and resveratrol could significantly improve these indicators.The results of histopathology showed that the number of seminiferous epitheli-um layers and the area of intercellular substance in the hind-limp unloading group were significantly re-duced,with obvious edema,and the number of spermatogonium was significantly reduced.After treat-ment with resveratrol,the morphology of testicular tissue was partially restored.The detection results of oxidative stress and inflammatory factor levels showed that compared with the placebo control group,the hind-limp unloading group showed an imbalance in oxidative stress levels in the testicular tissue of rats,with a significant increase in inflammatory factor levels.After treatment with resveratrol,this harmful situation could be significantly reversed.Western blot results showed that resveratrol signifi-cantly reduced the expression levels of Bax in the testicular tissue of hind-limp unloading male rats,and increased the expression levels of Bcl-2,p-PI3K/PI3K,p-AKT/AKT.Conclusions resveratrol of 30 mg·kg-1 may exert a antagonistic effect on reproductive damage in hind-limp unloading male rats by ac-tivating the PI3K/AKT signaling pathway.