食品工业科技2024，Vol.45Issue(7)：93-99,7.DOI:10.13386/j.issn1002-0306.2023050212
葡萄汁有孢汉逊酵母与酿酒酵母共培养发酵野樱桃饮品研究
Characteristics of Wild Cherry Beverage Co-fermented by Hanseniaspora uvarum and Saccharomyces cerevisiae
摘要
Abstract
One strain of Hanseniaspora uvarum YT-35 was screened from fermented sediment of wild cherry.Hanseniaspora uvarum YT-35 and commercial Saccharomyces cerevisiae were used as coculture for manufacture of fermented wild cherry beverage.The dynamics of microbial populations,reducing sugars and ethanol were analyzed at different stages of fermentation using single-strain fermentation with 2 strains of bacteria as a control.Meanwhile,the organic acids and volatile aromatic compounds of the fermented beverages were detected by high-performance liquid chromatography(HPLC)and headspace solid-phase microextraction/gas chromatography-mass spectrometry(HS-SPME/GC-MS).The results showed that H.uvarum YT-35 dominated in the pre-fermentation stage of co-culture.Compared with single fermentation with S.cerevisiae,the coculture fermentation resulted in lower ethanol content(3.51 g/L).Notably,HPLC results revealed that coculture fermented beverage reduced the yield of citric,malic and quinic acids and increased the yield of glacial acetic acid.HS-SPME/GC-MS results revealed that coculture fermented beverage produced more volatile compounds of esters,such as ethyl caproate,methyl benzoate and isoamyl octanoate and showed enhanced contents of ethyl laurate,ethyl octanoate,phenyl ethyl alcohol,benzyl alcohol,octanoic acid and lauric acid.Meanwhile,clustering analysis revealed that coculture fermentation were correlated with the greatest number of volatile aroma compounds in the fermented wild cherry beverage.This study provides scientific basis and theoretical guidance for the research of coculture strains with different metabolic potential in improving the quality of fruit juice fermented beverage.关键词
葡萄汁有孢汉逊酵母/酿酒酵母/共培养/野樱桃发酵饮品/挥发性成分Key words
Hanseniaspora uvarum/Saccharomyces cerevisiae/coculture/wild cherry fermented beverage/volatile compounds分类
轻工纺织引用本文复制引用
李婵媛,郑淼心,邹玉婷,贺紫涵,许碧涛,张庆,秦佳,田文强..葡萄汁有孢汉逊酵母与酿酒酵母共培养发酵野樱桃饮品研究[J].食品工业科技,2024,45(7):93-99,7.基金项目
四川省自然科学基金(2022NSFSC0105) （2022NSFSC0105）
四川省科技攻关项目(2022YFN0016). （2022YFN0016）
