食品工业科技2024，Vol.45Issue(7)：191-200,10.DOI:10.13386/j.issn1002-0306.2023050290
桑黄总黄酮的提取、组分分析及其生物活性评价
Extraction,Component Analysis and Biological Activity Evaluation of Total Flavonoids from Phellinus igniarius
摘要
Abstract
In order to study the extraction technology of total flavonoids from Phellinus igniarius systematically,clarify the composition and content of flavonoids in total flavonoids,and explore the biological activity of total flavonoids from Phellinus igniarius.The extraction technology of Phellinus igniarius total flavonoids was optimized by response surface method,the composition of Phellinus igniarius total flavonoids was analyzed,and some of its biological activities were also detected.The results showed that the optimal extraction conditions were extraction temperature of 73℃,solid-liquid ratio of 1:50 g/mL and extraction time of 3 h,under which the yield of Phellinus igniarius total flavonoids reached 2.68%.The types and contents of flavonoids in Phellinus igniarius total flavonoids were determined by HPLC.Total flavonoids of Phellinus igniarius mainly contained taxifolin,quercetin and kaempferol,and the content of taxifolin was 3727.31 mg/kg.The analysis of biological activities of Phellinus igniarius total flavonoids showed that it had certain antioxidant,lipid-lowering and hypoglycemic activities in vitro,and the scavenging rate of DPPH free radicals reached 58.63%±0.45%when its concentration was 14 μg/mL,and the scavenging rate of hydroxyl free radicals reached 52.51%±1.49%when its concentration was 0.08 mg/mL.The experimental results of lipid-lowering activity showed that the inhibitory rate of Phellinus igniarius total flavonoids on pancreatic lipase reached 10.56%±0.06%at the concentration of 6 mg/mL,and the inhibitory rate on cholesterol micelle solubility reached 32.59%±0.78%at the concentration of 4 mg/mL.Phellinus igniarius total flavonids had obvious hypoglycemic activity,and its IC50 for α-glucosidase and α-amylase was 71.42 mg/mL and 97.28 mg/mL respectively.关键词
桑黄/总黄酮/提取/组分分析/生物活性Key words
Phellinus igniarius/total foavonoids/extraction/component analysis/biological activity分类
生物科学引用本文复制引用
付婷伟,周紫微,邵颖,张朋,宁晓雅,杨洋,陈安徽..桑黄总黄酮的提取、组分分析及其生物活性评价[J].食品工业科技,2024,45(7):191-200,10.基金项目
江苏省科技计划(XZ-SZ202150) （XZ-SZ202150）
徐州市科技计划重点研发项目(KC20047). （KC20047）
