食品工业科技2024，Vol.45Issue(7)：244-253,10.DOI:10.13386/j.issn1002-0306.2023060295
响应面优化硒化白及多糖的制备工艺及其体外抗氧化活性研究
Optimization of Selenized Bletilla striata Polysaccharides Preparation by Response Surface Methodology and Determination of the Antioxidant Activity in Vitro
摘要
Abstract
In this study,the preparation process of selenized Bletilla striata polysaccharides(BSP-Se)was optimized,to explore whether higher antioxidant activity preparation could be obtained.BSP-Se was prepared by using Bletilla striata polysaccharide(BSP)as the raw material,Na2SeO3 as the selenifying agent,and acetic acid as the catalyst.Taking selenium content as an indicator,the preparation process was optimized using single factor experiments and response surface methodology.Fourier-transform infrared spectroscopy was used to determine whether BSP-Se was successfully prepared,and the antioxidant activity of BSP-Se in vitro was evaluated by measuring the scavenging ability of DPPH,ABTS+ and·OH free radicals.The results showed that the selenium content of BSP-Se prepared under the optimal process conditions of Na2SeO3 to BSP mass ratio of 1.5,acetic acid to BSP dosage ratio of 3,reaction time of 6 hours,and reaction temperature of 80℃was the highest,reaching 7.831 mg/g.FT-IR analysis showed that selenium binds to BSP through Se-O-C and O-Se-O,indicating that the polysaccharides have been successfully selenified.The analysis of free radical scavenging ability showed that the IC50 values of BSP-Se for DPPH,ABTS+,and·OH free radicals were 2.875,4.431 and 0.314 mg/mL,respectively,which were significantly lower than the IC50 values of 5.828,8.475 and 0.833 mg/mL of BSP(P＜0.05),indicating that selenization modification improved the antioxidant capacity of BSP.Therefore,the antioxidant activity of BSP can be improved by selenization modification,which provides a theoretical basis for further research on the application of selenium polysaccharides as a new antioxidant in the fields of foods and medicine,health care products and daily chemical.关键词
白及多糖/硒化改性/响应面优化/制备工艺/抗氧化活性Key words
Bletilla striata polysaccharide/selenide modification/response surface optimization/preparation process/antioxidant activity分类
轻工纺织引用本文复制引用
涂玲飞,李焱,张振..响应面优化硒化白及多糖的制备工艺及其体外抗氧化活性研究[J].食品工业科技,2024,45(7):244-253,10.基金项目
两种地理标志产品特征指标评价及应用研究(黔科院R字[2022]01号). （黔科院R字[2022]01号）
