乳业科学与技术2023，Vol.46Issue(6)：20-24,5.DOI:10.7506/rykxyjs1671-5187-20231224-062
基于实时荧光定量技术建立定量检测乳粉中羊源性成分的方法
Establishment of a Fluorescence-Based Quantitative Real-Time PCR Method for Detection for Caprine-Derived Ingredients in Milk Powder
摘要
Abstract
This study established a fluorescence-based quantitative real-time polymerase chain reaction method for the detection of caprine-derived ingredients in milk powder.The specificity,sensitivity,and stability of the caprine-specific primers were evaluated.By linear fitting of the difference in cycle threshold(ΔCt)as a function of the mixing proportion between goat and horse milk powder,a calibration curve for the relative quantitation of caprine-derived ingredients in milk powder was established as follows:y = 0.720 9x + 5.651 9(R² = 0.987 5).The recoveries of the proposed method were 96.22%-112.00%,and the inter-and intra-group coefficient of variation was≤0.79%and≤1.77%,respectively.关键词
羊乳粉/马乳粉/实时荧光定量聚合酶链式反应/掺假检测Key words
goat milk powder/horse milk powder/real-time fluorescence quantitative polymerase chain reaction/adulteration detection分类
轻工纺织引用本文复制引用
陈晨,张瑞,王玉欣,张同贺,张子仑,贾文珅,周巍..基于实时荧光定量技术建立定量检测乳粉中羊源性成分的方法[J].乳业科学与技术,2023,46(6):20-24,5.基金项目
河北省市场监督管理局科研计划项目(2022ZD11) （2022ZD11）
