乳业科学与技术2023，Vol.46Issue(6)：29-33,5.DOI:10.7506/rykxyjs1671-5187-20240116-003
超高效液相色谱法同时测定配方乳粉中的烟酸、烟酰胺和VB6
Simultaneous Determination of Niacin,Nicotinamide,and Vitamin B6 in Formula Milk Powder by Ultra Performance Liquid Chromatography
崔苏霞 1孟根花 1蔺婷娟 1李晓燕1
作者信息
- 1. 内蒙古伊利实业集团股份有限公司,内蒙古 呼和浩特 010110
- 折叠
摘要
Abstract
To establish an ultra performance liquid chromatography(UPLC)analysis method that can simultaneously determine niacin,niacinamide,and vitamin B6 in formula milk powder.Gradient elution was performed with 10mmol/L ammonium acetate solution(containing 0.1%formic acid)as mobile phase A and methanol as mobile phase B.The analytes were separated by Poroshell 120 EC-C18 in tandem with ACQUITY UPLC HSS T3 column,detected with the UV detector and fluorescence detector,and quantified by an external standard method.Good linearity was observed for niacin concentrations in the range of 0.0-0.8 µg/mL,nicotinamide concentrations in the range of 0.0-20.0 µg/mL,pyridoxamine concentrations in the range of 0.0-0.2 µg/mL,pyridoxal concentrations in the range of 0.0-0.6 µg/mL and pyridoxine concentrations in the range of 0.0-4.0 µg/mL,and the correlation coefficients of standard curves were all greater than 0.999.The limits of detection and quantification met the requirements.The average recoveries of the method were all in the range of 80.5%-104.1%with relative standard deviation(RSD)were 0.37%-2.99%.This method is simple,accurate,and effectively saves detection costs by about 33%,improving detection efficiency by about 4 times,It can be used for batch detection of niacin,niacinamide,and vitamin B6 in formula milk powder.关键词
配方乳粉/烟酸/烟酰胺/VB6/超高效液相色谱法/检测Key words
formula milk powder/Niacin/niacinamide/vitamin B6/ultra performance liquid chromatography/determination分类
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崔苏霞,孟根花,蔺婷娟,李晓燕..超高效液相色谱法同时测定配方乳粉中的烟酸、烟酰胺和VB6[J].乳业科学与技术,2023,46(6):29-33,5.
