乳业科学与技术2023，Vol.46Issue(6)：34-39,6.DOI:10.7506/rykxyjs1671-5187-20231201-058
超高效液相色谱-串联质谱法测定牛乳和乳粉中伏马毒素残留
Quantitative Determination of Fumonisin Residues in Milk and Milk Powder by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
An ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method for the simultaneous quantitative determination of fumonisin B1(FB1),fumonisin B2(FB2)and fumonisin B3(FB3)residues in milk powder and milk has been established.The samples were extracted with methanol-acetic acid(98:2,V/V),diluted,cleaned up by immunoaffinity chromatography(IAC),and blown to dryness under nitrogen.The residue was re-dissolved and detected using an electrospray ionization source(ESI)in the positive ion mode with multiple reaction monitoring(MRM)and quantitative analysis was performed by the isotope-labeled internal standard method.The limit of quantification was 10 μg/kg for FB1,FB2 and FB3 in milk powder and milk.The recoveries at spiked concentration levels of 10-400 μg/kg were 92.8%-107.5%,with relative standard deviations(RSD)of 2.0%-4.3%.The matrix effects were 96.25%-122.84%.This method was characterized by short analysis time and was suitable for the determination of fumonisin residues in different dairy products.关键词
超高效液相色谱-串联质谱/伏马毒素类/真菌毒素/婴幼儿食品/乳粉/牛乳Key words
ultra-high performance liquid chromatography-tandem mass spectrometry/fumonisins/mycotoxin/infant food/milk powder/milk分类
轻工纺织引用本文复制引用
莫楠,张立佳,高玉杰,白艳梅,汪洋,刘丽君,李翠枝..超高效液相色谱-串联质谱法测定牛乳和乳粉中伏马毒素残留[J].乳业科学与技术,2023,46(6):34-39,6.基金项目
内蒙古自治区重点研发和成果转化计划项目(2023YFHH0093) （2023YFHH0093）
