乳业科学与技术2023，Vol.46Issue(6)：40-43,4.DOI:10.7506/rykxyjs1671-5187-20231011-052
超高效液相色谱检测乳及乳制品中柠檬酸的含量
Determination of the Content of Citric Acid in Milk and Dairy Products by Ultra-High Performance Liquid Chromatography
徐红 1唐振鑫 1张雪娇 1冀何连 1赵姝丽 1王忱 1高万东 1吴晓莉 1张彩霞1
作者信息
- 1. 内蒙古伊利实业集团股份有限公司,内蒙古 呼和浩特 010110
- 折叠
摘要
Abstract
An ultra-high performance liquid chromatography(HPLC)method was established for the determination of the content of citric acid in dairy products.The samples were precipitated by K3Fe(CN)6 and(CH3COO)2Zn,extracted ultrasonically,and cryogenically centrifuged to precipitate protein and fat.The chromatographic separation was accomplished on a Poroshell 120 Aq-C18 column(4.6 mm×100 mm,2.7 μm)using gradient elution with a mobile phase consisting of a mixture of methanol and ammonium dihydrogen phosphate solution.The analyte was detected by an ultraviolet(UV)detector and quantified by the external standard method.The results showed that the calibration curve was linear with a correlation coefficient above 0.999 9.The average recovery from spiked samples was 95.3%-104.8%,the detection limit was 100 mg/kg,and the relative standard deviation(RSD)for precision was 0.16%-2.41%.The method is simple and rapid and has good separation effect.It is suitable for the quantitative analysis of citric acid in milk and dairy products.关键词
柠檬酸/超高效液相色谱/灭菌乳/发酵乳/乳制品Key words
citric acid/ultra-high performance liquid chromatography/sterilized milk/fermented milk/dairy products分类
轻工纺织引用本文复制引用
徐红,唐振鑫,张雪娇,冀何连,赵姝丽,王忱,高万东,吴晓莉,张彩霞..超高效液相色谱检测乳及乳制品中柠檬酸的含量[J].乳业科学与技术,2023,46(6):40-43,4.
