食品工业科技2024，Vol.45Issue(7)：8-14,7.DOI:10.13386/j.issn1002-0306.2023070216
复合酶法提取槐花多糖的工艺优化及其抗氧化活性
Optimization of Extraction Process of Polysaccharide from Sophora japonica by Compound Enzyme Method and Its Antioxidant Activity
摘要
Abstract
Objective:Sophora japonica polysaccharides were extracted by compound enzyme method,and the extraction process was optimized.The antioxidant activity in vitro was evaluated.Methods:The effects of addition amount of compound enzyme,pH,proportion of compound enzyme and enzymatic hydrolysis time on the extraction yield were investigated by single factor experiment.On the basic of single factor experiment,response surface method was used to determine the optimal extraction parameters of Sophora japonica polysaccharide.Compared with VC,the antioxidant activity of Sophora japonica polysaccharides was investigated by measuring the scavenging rate of DPPH·and ABTS+·and the total reducing power.Results:The optimal extraction parameters of Sophora japonica polysaccharides were as follows:The addition amount of compound enzyme was 23.8 mg/g,pH4.8,and the ratio of pectinase to cellulase was 0.912:1.Under this process,the yield of Sophora japonica polysaccharides was 10.71%,and the extracted polysaccharide showed good scavenging ability for DPPH·and ABTS+·.When the concentration of the polysaccharide solution was 2.8 mg/mL,the scavenging rate of DPPH·and ABTS+·reached 94.19%and 99.79%of VC at the same concentration,respectively,and the total reducing power reached 75.99%of VC.Conclution:Sophora japonica polysaccharide could be effectively extracted by compound enzymatic method and its antioxidant activity could be improved,which provided a theoretical reference for the development of functional food of Sophora japonica polysaccharide.关键词
槐花/多糖/复合酶/提取/抗氧化Key words
Sophora japonica/polysaccharides/compound enzyme/extraction/antioxidant分类
轻工纺织引用本文复制引用
任晓莉,杨璐,乔鹏,缪奕锴,杨懿昂,代秋红,张贤德..复合酶法提取槐花多糖的工艺优化及其抗氧化活性[J].食品工业科技,2024,45(7):8-14,7.基金项目
大学生创新创业项目(GK2022131,202314101022). （GK2022131,202314101022）
