藁本内酯抑制缺氧缺血性脑损伤新生大鼠脑组织凋亡相关蛋白的表达OACSTPCD

Objective:To evaluate the neuroprotective effect of ligustilide(LGSL)on neonatal rats with hypoxic ischemic brain damage(HIBD)by measuring NOD-like receptor protein 3(NLRP3),aspartate proteolytic enzyme 1(Caspase 1),interleukin-1β(IL-1β)and GSDMD-N protein levels induced by ischemia and hypoxia in vivo and in vitro.Methods:Seven-day-old male SD rats were randomly divided into the Sham-operated group(Sham group),HIBD group,LGSL group,with 10 rats in each group.The HIBD model was constructed by double ligation of the left common carotid artery,and the microglia oxygen-glucose deprivation(OGD/R)model was established in vitro.CCK-8 was used to evaluate the effects of different concentrations of LGSL on microglia activity after OGD/R stimulation for 24 h.Effects of 20 μM LGSL on expression of NLRP3,Caspase 1,IL-1 β and GSDMD-N were evaluated by Western blotting.Results:Expressions of NLRP3,IL-1β,Caspase 1 and GSDMD-N were all up-regulated in vivo and in vitro(P＜0.05).After 20 μM LGSL intervention for 24 h,the expressions of NLRP3,IL-1β,Caspase 1 and GSDMD-N were down-regulated(P＜0.05).Conclusion:LGSL can significantly inhibit neuroinflammatory responses induced by ischemia and hypoxia both in vivo and in vitro.