临床口腔医学杂志2024，Vol.40Issue(3)：131-135,5.DOI:10.3969/j.issn.1003-1634.2024.03.002
薯蓣皂苷对口腔鳞癌细胞增殖和凋亡的影响
Effect of dioscin on proliferation and apoptosis of oral squamous cell carcinoma cells
摘要
Abstract
Objective:To investigate the effect of dioscin on the proliferation and apoptosis of human oral squamous cell carcinoma cells SCC-4 and SCC-25.Methods:SCC-4 and SCC-25 cells were treated with different concentrations(0,0.1,0.3,1,3,10,30 µmol/L)of dioscin for 24 h,48 h and 72 h.The effect of dioscin on the proliferation of SCC-4 and SCC-25 cells was detected by CCK-8 method,and the subsequent experimental concentrations(0,1,2,4 µmol/L)were select-ed according to the IC50 value.EdU was used to detect the effect of dioscin on the proliferation of SCC-4 and SCC-25 cells.The effect of dioscin on the apoptosis of SCC-4 and SCC-25 cells were detected by flow cytometry.The expression levels of Noxa and Cleaved-Caspase 3 proteins in cells treated with dioscin for 48 h were detected by Western Blot.The mRNA expres-sion of Noxa in cells was detected by qRT-PCR.Results:Within a certain concentration range,dioscin could inhibit the pro-liferation of oral squamous cell carcinoma cells SCC-4 and SCC-25 in a time-dependent and dose-dependent manner(P＜0.05).At the same time,the experimental group(1,2,4 μmol/L)dioscin could promote the apoptosis of SCC-4 and SCC-25 cells after 48 h intervention(P＜0.05),and up-regulate the expression levels of Noxa and Cleaved-Caspase 3 in SCC-4 and SCC-25 cells,and the mRNA expression of Noxa in SCC-4 and SCC-25 cells increased with the concentration(P＜0.05).Conclusion:A certain concentration of dioscin can effectively inhibit the proliferation of oral squamous cell carcinoma cells and induce the apoptosis of oral squamous cell carcinoma cells.The mechanism may be related to the up-regulation of the ex-pression of pro-apoptotic protein Noxa by dioscin.关键词
薯蓣皂苷/口腔鳞状细胞癌/凋亡/NoxaKey words
Dioscin/Oral squamous cell carcinoma/Apoptosis/Noxa分类
医药卫生引用本文复制引用
张慧琳,章作号,龚伶玲,王小聪,乐曼妮,黄子璇,李明,张睿..薯蓣皂苷对口腔鳞癌细胞增殖和凋亡的影响[J].临床口腔医学杂志,2024,40(3):131-135,5.基金项目
湖南省自然科学基金面上基金项目(编号2022JJ30630) （编号2022JJ30630）
湖南省教育厅重点项目(编号22A0249) （编号22A0249）
长沙市自然科学基金项目(编号kq2208458) （编号kq2208458）
湖南省科技厅临床医疗技术创新引导项目(编号2021SK53301) （编号2021SK53301）
湖南省卫生健康委员会重点指导课题(编号202108051626) （编号202108051626）
