香苏杂交猪ARHGAP24基因SNP检测及生长性状关联分析OA北大核心CSTPCD

RhoGTPase activating protein 24(ARHGAP24)gene is closely related to actin remodeling,cell polarity control and cell migration,and this study aims to explore the relationship between ARHGAP24 gene polymorphisms and the growth traits of Xiangsu hybrid pigs Sus scrofa,so as to provide a reference for the molecular marker selection of Xiangsu hybrid pigs.In this study,the blood DNA of 164 Xiangsu hybrid pigs under the same feeding conditions was collected,and the SNP sites of ARHGAP24 genes were determined by PCR amplification and sequencing.The general linear model in SPSS 25.0 software was used to analyze the association between the SNP site of the ARHGAP24 gene and the growth traits of Xiangsu hybrid pigs.A total of 5 SNP sites were detected in 9 exons of Xiangsu hybrid pigs,located in exon 1 and exon 8,respectively.g.735024 A>G nonsynonymous mutations caused valine at position 455 to change to aspartic acid on mRNA,which had no obvious effect on the structure of mRNA,but leaded to the α-helix and irregular coil of the protein secondary structure,and the decrease of β-corner and extension chain.After the nonsynonymous mutation of the g.735242 A>C site,the 528th position valine changed to serine,which produced obvious changes in the mRNA structure,while the proportion of α-helix and extended chain in the secondary structure of the protein decreased slightly,and the proportion of β-turn angle and irregular curl increased slightly.The analysis of association results showed that the C>T locus of g.735313 had significant effect on chest circumference(P＜0.01)and abdominal circumference(P＜0.05).Linkage imbalance analysis of 5 SNPs sites detected 5 haplotypes and 15 doubles.The g.735313 C>T loci in the ARHGAP24 gene of Xiangsu hybrid pigs were significantly associated with the chest and abdominal circumference of Xiangsu hybrid pigs,and were moderately polymorphic and in Hardy-Weinberg equilibrium,which could be used as candidate sites for molecular breeding of genetic markers of Xiangsu hybrid pigs in the later stage.