基于PDS基因家族的海马齿VIGS体系的构建OA北大核心CSTPCD

Sesuvium portulacastrum is a succulent saline plant,which is extremely tolerant to high salt stress.Virus-induced gene silencing(VIGS)is an important research tool to characterise the gene function of plants.In order to establish an efficient VIGS system in the important salt-tolerant plant S.portulacastrum,phytoene desaturase(PDS)gene was selected as a marker gene using S.portulacastrum of Sanya as the material.According to the transcriptome data,5 PDS family members of S.portulacastrum were identified by local Blast and other biosignature means,and the common sequences of the 5 members were obtained by PCR,with a length of 431 bp.Meanwhile,SpTRV2-PDS silencing expression vector was constructed,which was transformed by Agrobacterium tumefaciens GV3101 and then infiltrated into the plants by injection.The phenotype was observed,and the changes of carotenoid content and PDS gene expression were measured.The results showed that after infection,the plants showed slow growth and gradual yellowing of leaves,and the leaves showed albino phenomenon after 7 d,and the carotenoid content and the relative expression of PDS in the experimental group continued to exist,indicating that the VIGS system of S.portulacastrum was successfully constructed,indicating that the VIGS system could effectively silence the expression of PDS gene in S.portulacastrum.This study provides a technical means for the identification of the function of salt tolerance genes in S.portulacastrum.