上海海洋大学学报2024，Vol.33Issue(2)：297-307,11.DOI:10.12024/jsou.20230304116
雌性三角帆蚌Srd5a1基因的分子鉴定和功能研究
Molecular identification and functional study of Srd5a1 in female Hyriopsis cumingii
摘要
Abstract
Sex steroid hormones are closely related to biological reproduction,gamete emission and gonadal development.And Srd5a1 plays an important role in the synthesis of sex steroid hormones.In order to investigate the effect of the sex steroid hormone synthesis-related enzyme gene Srd5a1 on ovarian development in female Hyriopsis cumingii,Srd5a1 was obtained from the gonads of H.cumingii by RACE(Rapid-amplificiantion of cDNA ends).The expression characteristics of Srd5a1 were investigated by real-time fluorescence quantification(qRT-PCR),in situ hybridization and in vitro induction of 17β-estradiol(E2)and 17α-methyltestosterone(MT)at different concentrations for 24 days.The results showed that the full-length cDNA of Srd5a1 was 2 224 bp,including ORF 825 bp,3'-UTR 953 bp,and 5'-UTR 446 bp,encoding 274 amino acids.The expression of Srd5a1 in the gonad was bimodal,with higher expression in the ovary than that in the testis;Srd5a1 had the highest expression level in the ovarian discharge period,which was extremely significantly higher than that in all other periods.In situ hybridization results showed that Srd5a1 detected positive signals in ovarian oocytes and follicles as well as spermatocytes of the testis.The expression of Srd5a1 was changed after 24 days of treatment with different concentrations of E2 and MT.In conclusion,Srd5a1 may play a role in the developmental maturation of the ovary oocyte and gamete emission in the female H.cumingii.This study is important for exploring the gonadal growth and development of H.cumingii,and also provides a theoretical reference value for the realization of monogamous breeding of H.cumingii.关键词
三角帆蚌/Srd5a1基因/卵巢发育/17β-雌二醇/17α-甲基睾酮Key words
Hyriopsis cumingii/Srd5a1/ovarian development/17β-estradiol/17α-methyltestosterone分类
农业科技引用本文复制引用
曹慕莲,霍滢朵,刘宗雨,缪煜琳,金鑫,汪桂玲..雌性三角帆蚌Srd5a1基因的分子鉴定和功能研究[J].上海海洋大学学报,2024,33(2):297-307,11.基金项目
国家重点研发计划"蓝色粮仓科技创新"项目(2022YFD2400700) （2022YFD2400700）
国家自然科学基金(31772835) （31772835）
财政部和农业农村部国家现代农业产业技术体系项目(CAR-49) （CAR-49）
