上海海洋大学学报2024，Vol.33Issue(2)：341-351,11.DOI:10.12024/jsou.20230604260
雅罗鱼杂交种及其双亲耐碱性能相关生理指标比较
A comparative study on physiological indexes related to alkali resistance of Leuciscus hybrid and its parents
摘要
Abstract
To investigate the alkali resistance of the hybrid of Leuciscus and its parent Leuciscus waleckii(female parent)and Leuciscus idus(male parent)of the Irtysh River in Xinjiang,the alkalinity stress experiment of 50 mmol/L NaHCO3 was carried out on three experimental fish for 7 days.Several physiological indexes and corresponding gene expressions involving osmotic pressure regulation and ammonia metabolism and significantly related to alkali resistance were compared and studied.The results showed that the Na+/K+ ATPase(NKA)activity and gene expression(ATPase α1-like and ATPase α3)of the gill tissues of the female parent with strong alkali resistance were higher than those of hybrids,and significantly higher than those of the male parent.The serum alkaline phosphatase(AKP)activity and the gene expression amount(alpl)of gill tissue were significantly lower than those of the other two fish,indicating that the female parent may regulate NKA enzyme activity through AKP dephosphorylation and participate in osmotic pressure regulation under high alkaline stress.The detection of ammonia metabolism related indexes showed that the blood ammonia(Amon)and urea nitrogen(BUN)contents of the female parent were extremely significantly lower than those of the other two fish,while the serum glutamate dehydrogenase(GDH)and glutamine synthetase(GS),as well as the expression of related genes in the gill tissue(glud,glul,Rhbg and Rhcg1)were mostly higher than those of hybrids,and significantly higher than those of the male parent,indicating that the female parent consumed ammonia in the body by synthesizing ammonia intermediate metabolites and increasing the activity of ammonia transporters.In summary,these physiological indexes were significantly related to the female parent's strong alkali resistance,and were well inherited to its hybrid offspring.The results of this study provide a theoretical basis for the comprehensive evaluation of the alkali resistance of fish,and lay a working foundation for the selection and breeding of new varieties of alkali resistance and the declaration of improved varieties of Leuciscus.关键词
雅罗鱼/杂交种/耐碱性能/渗透压调节/氨代谢Key words
Leuciscus/hybrids/alkali resistance/osmotic pressure regulation/ammonia metabolism分类
农业科技引用本文复制引用
李雯,艾克达·热合曼,邹宇婷,黄晶,张立民,常玉梅..雅罗鱼杂交种及其双亲耐碱性能相关生理指标比较[J].上海海洋大学学报,2024,33(2):341-351,11.基金项目
国家重点研发计划(2020YFD0900402) （2020YFD0900402）
国家自然科学基金(32273120) （32273120）
