改善肿瘤缺氧耐药的血红蛋白-紫杉醇脂质体的制备及体外评价OACSTPCD

Objective To prepare liposomes encapsulate hemoglobin and paclitaxel(LEHP)to improve tumor hypoxia resistance.Methods LEHP were prepared by thin-film method,and the particle size,Zeta potential and polydispersity were investigated by nanoparticle size analyzer,and encapsulation efficiency was investigated by high performance liquid chromatography,and the interaction between the liposomes and tumor cells was evaluated by in vitro cell experiments.Re-sults The optimal preparation conditions of LEHP was as follows:total phospholipid 36 mM,DPPC:Dope:cholesterol mo-lar ratio 7:2:1,paclitaxel 3 mg,hydrated with 3 mg·mL-1 Hb-PBS for 30 min at room temperature;The average particle size was(189.17±8.22)nm,polydispersity was 0.14±0.023,paclitaxel encapsulation efficiency was(58.27±2.55)％,he-moglobin content was(0.63±0.05)mg·mL-1.In vitro cell experiments,the killing effect of LEHP was about 1.5 times that of LEP,about 1.2 times that of LEP,and ROS production was about 1.8 times that of LEP.Conclusion The preparation conditions of LEHP was optimized,and cell experiments showed that LEHP can promote tumor cell apoptosis by improving hypoxia and increasing ROS production,which is expected to provide a safe and effective new method for drug resistance caused by tumor hypoxia.