海南医学院学报2024，Vol.30Issue(6)：442-450,9.DOI:10.13210/j.cnki.jhmu.20231106.001
肝细胞癌干性分型及其预后基因GSDMC的研究
Study on stemness typing and prognostic gene GSDMC of hepatocellular carcinoma
摘要
Abstract
Objective:To screen the patients with hepatocellular carcinoma who are more responsive to immunotherapy by stemness typing,and to verify the prognosis related gene GSDMC.Methods:Twenty-six stem cell genomes were extracted from the StemChecker database,consensus clustering algorithm was applied for the identification of stemness subtypes on 328 hepato-cellular carcinoma samples from The Cancer Genome Atlas Program databases.The differences in prognosis,tumor microenviron-ment components,and treatment response between the two subtypes were assessed.Then,a stem risk model was constructed by integrating weighted gene correlation network analysis,Cox regression and random survival forest analyses.Finally,the prognostic-related gene GSDMC was selected for experimental validation.Results:Based on single-sample gene set enrichment analysis enrichments scores,hepatocellular carcinoma patients were classified into two subtypes(C1 and C2).There were signifi-cant differences in survivalrate,the levels of immune infiltration,and treatment reponse between C1 and C2.Integrating WGC-NA,Cox regression and random forest survival analyses identified four prognostic-related genes(GSDMC,AOC1,OTX1,CASC9).Immunohistochemistry and real-time-quantitative polymerase chain reaction showed that the expression of GSDMC was decreased in hepatocellular carcinoma tissues and cells.Sphere formation assay showed that GSDMC suppressesed hepatocellular carcinoma cells stemness.Conclusion:The identification of two distinct stem cell subtypes,C1 and C2,provides valuable insights into the clinical heterogeneity of hepatocellular carcinoma and its correlation with prognosis,tumor microenvironment characteris-tics,and immunotherapy response rates.The expression of GSDMC is decreased in hepatocellular carcinoma tissues and cells,and GSDMC inhibits the stemness of hepatocellular carcinoma cells.关键词
肝细胞癌/GSDMC/干性/肿瘤微环境/免疫治疗Key words
Hepatocellular cacinoma/GSDMC/Stemness/Tumor microenvironment/Immunotherapy分类
医药卫生引用本文复制引用
包凌,龚旋坤,庞青,陈晓..肝细胞癌干性分型及其预后基因GSDMC的研究[J].海南医学院学报,2024,30(6):442-450,9.基金项目
This study was supported by Anhui Provincial Natural Science Foundation Project(2008085J37) 安徽省自然科学基金资助项目(2008085J37) （2008085J37）
