1株植物内生菌解淀粉芽孢杆菌的安全性评价OACSTPCD
Safety evaluation of a Bacillus amyloliquefaciens form plant endophytic bacterium
试验旨在评价1株从杜仲中分离的解淀粉芽孢杆菌的安全性.试验从菌株溶血性、氨基酸脱羧和明胶液化试验、抗菌药物敏感性、腹腔注射、经口灌胃、抑菌活性等进行安全性综合评价.腹腔注射试验分为腹腔注射组(1.0×1010 CFU/0.3 mL)和生理盐水组,一次性注射,连续观察5d.经口灌胃试验分为高剂量组(1.0×1010 CFU/0.2mL)、中剂量组(1.0× 109 CFU/0.2 mL)、低剂量组(1.0×108 CFU/0.2 mL)和生理盐水组,每天灌胃1次,连续灌胃28 d.结果显示,腹腔注射和经口灌胃小鼠均健康存活,未见临床变化,试验期间体重正常增加,各组小鼠血常规、血清生化指标和脏器指数差异均不显著(P>0.05),解淀粉芽孢杆菌DZ01未发生易位,氨基酸脱羧和明胶液化试验均为阴性.解淀粉芽孢杆菌DZ01对所测的8种抗菌药物均表现敏感.除粪肠球菌和铜绿假单胞菌外,解淀粉芽孢杆菌DZ01上清液对其余6株指示菌均具有抑菌活性.研究表明,解淀粉芽孢杆菌DZ01具有较好的安全性.
The experiment aimed to evaluate the safety of a Bacillus amyloliquefaciens isolated from Eucommia ulmoides.Safety assessments were conducted through comprehensive evaluations of hemolysis,amino acid decarboxylation,gelatin liquefaction tests,antibiotic sensitivity,intraperitoneal injection,oral gavage,and antimicrobial activity.The intraperitoneal injection experiment included an injection group(1.0 × 1010 CFU/0.3 mL)and a normal saline group,with a single injection and continuous observation for five days.The oral gavage experiment comprised a high-dose group(1.0 × 1010 CFU/0.2 mL),a medium-dose group(1.0 × 109 CFU/0.2 mL),a low-dose group(1.0 × 108 CFU/0.2 mL),and a normal saline group,with daily gavage for 28 days.The results showed that both intraperitoneal injection and oral gavage mice exhibited healthy survival,with no clinical changes observed.During the experimental period,the body weight increased normally,and there were no significant differences in blood routine,serum biochemical indicators,and organ indices among the groups(P>0.05).Bacillus strain DZ01 did not exhibit translocation,and hemolysis,amino acid decarboxylation,and gelatin liquefaction tests were all negative.Bacillus strain DZ01 showed sensitivity to all eight tested antibiotics.Apart from Enterococcus faecalis and Pseudomonas aeruginosa,the supernatant of Bacillus strain DZ01 exhibited inhibitory activity against the other six indicator strains.The study suggests that Bacillus strain DZ01 has good safety.
吕文亮;白冬红;林显华;张甜甜;李永振;徐海燕;谷巍
山东宝来利来生物工程股份有限公司山东省动物微生态制剂重点实验室,山东泰安 271017
畜牧业
解淀粉芽孢杆菌安全性植物内生菌
Bacillus amyloliquefacienssafetyendophytic bacteria in plants
《饲料研究》 2024 (004)
85-91 / 7
山东省重点研发计划项目(项目编号:2022CXPT039)
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