不同水分管理栽培方式对山栏稻根际土壤细菌群落的影响OA北大核心CSTPCD
Impact of Different Water Management Cultivation Methods on the Rhizosphere Bacteria Community of Shanlan Upland Rice
[目的]山栏稻的栽培方式单一,传统刀耕火种的种植手段破坏环境,水作是山栏稻栽培的新模式.研究山栏稻在大田水作环境下根际微生物的变化情况.[方法]设置正常灌溉和干旱管理的山栏稻栽培处理,分别对山栏稻根际土壤细菌群落进行 16S rRNA基因扩增序列测定,测序数据结合土壤理化性质进行综合分析.[结果]栽培方式的改变明显影响了根际细菌群落的组成.正常灌溉处理的Nitrosprota和Proteobacteria相对丰度低于干旱管理,Firmicutes的相对丰度高于干旱管理.相对丰度前 10的细菌门群落与土壤理化性质具有相关性.相关性网络分析显示,正常灌溉处理中更多的细菌群落具有相互作用,正常灌溉处理拥有更复杂的细菌网络.[结论]不同水分管理栽培方式的山栏稻根际细菌群落组成发生显著变化,Nitrospirota、Proteobacteria细菌类群可能通过促进水稻根部吸收氮素来增强山栏稻抗逆性.正常灌溉处理中更多细菌群落相互作用,增强了生态系统的稳定性.
[Objective]The cultivation way of Shanlan upland rice is single,and the traditional slash and burn cultivation method damages the environment.Cultivation in water can be a new way for Shanlan upland rice.This work is to explore the changes in rhizosphere microorganisms of Shanlan upland rice under water cultivation environment in large fields.[Method]The cultivation treatment of Shanlan upland rice was set up with normal irrigation and drying management,and 16S rRNA gene amplification sequencing was performed on the bacterial community in the rhizosphere soil of Shanlan upland rice.The sequencing data was combined with soil physicochemical properties for comprehensive analysis.[Result]The changes in cultivation methods have significantly affected the composition of rhizosphere bacterial communities.In particular,the relative abundance of Nitrosprota and Proteobacteria in the normal irrigation is lower than that in the drying management,and the relative abundance of Firmicutes is higher than that in drying management.The top 10 bacterial communities with relative abundance are correlated with soil environmental factors.The correlation network analysis shows that more bacterial communities interact in normal irrigation,and there is a more complex bacterial network under normal irrigation.[Conclusion]Significant changes in bacterial community composition occure in the rhizosphere of Shanlan upland rice under different water management cultivation methods.The bacteria groups of Nitrospirota and Proteobacteria may improve the stress resistance of Shanlan upland rice by promoting nitrogen uptake at the root of rice.In normal irrigation,more bacterial community interactions enhance the stability of the ecosystem.
刘佳宁;李梦;杨新森;吴伟;裴新梧;袁潜华
海南大学热带农林学院,海口 570228中国农业科学院生物技术研究所,北京 100089
山栏稻栽培方式根际微生物土壤理化性质
Shanlan upland ricecultivation methodsrhizosphere microorganismssoil physicochemical properties
《生物技术通报》 2024 (003)
242-250 / 9
海南省重点研发项目(ZDYF2022XDNY182),海南省重大科技计划(ZDKJ202002-03)
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