生物技术通报2024，Vol.40Issue(3)：296-304,9.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0902
肠侵袭性大肠埃希菌噬菌体DK-13的生物学特性及应用
Biological Characteristics and Application of Enteroinvasive Escherichia coli Phage DK-13
摘要
Abstract
[Objective]Selection and isolation of phage that can lyse Enteroinvasive Escherichia coli(EIEC),analyzing its biological characterization,and investigating its sterilizing effect on contaminated pork.[Method]The double-layer agar method was used to isolate phages,and then the optimal multiplicity of infection,one-step growth curve and other biological characteristics of the isolate were determined.The genetic genomes were sequenced and analyzed,and the lysis efficiency was evaluated.[Result]A bacteriophage that specifically lysed EIEC was isolated from hospital sewage and named DK-13.It has a typical tadpole-like morphology,consisting of a head and a contractile helical symmetric tail.It belongs to the family Myoviridae.The biological characterization showed that the optimal multiplicity of infection was 0.01,the incubation period was about 10 min,the lysis period was about 70 min,and the phage DK-13 was able to survive at 50℃and pH 5.0-10.0.The whole genome sequencing showed that the phage genome was about 172 275 bp in length,with a GC content of 40.18%,and was predicted to have a total of 293 open reading frames(ORFs),and no resistance genes or virulence genes were found.the sterilization effect in the contaminated pork was good,and the number of host bacteria significantly reduced.[Conclusion]A new virile phage DK-13 was isolated and identified,which has the advantages of short incubation period and high cleavage efficiency,and has great potential for application in food safety.关键词
肠侵袭性大肠埃希菌/噬菌体/噬菌体疗法/食品安全Key words
Enteroinvasive Escherichia coli/phage/phage application/food safety引用本文复制引用
王梦雅,刘家齐,姜海霖,李菁华,赵春燕,黄红兰..肠侵袭性大肠埃希菌噬菌体DK-13的生物学特性及应用[J].生物技术通报,2024,40(3):296-304,9.基金项目
吉林省科技厅国际合作项目(20210402023GH) （20210402023GH）
