山东农业科学2024，Vol.56Issue(3)：1-10,10.DOI:10.14083/j.issn.1001-4942.2024.03.001
高粱SBP基因家族鉴定及干旱胁迫下的表达模式分析
Identification of Sorghum SBP Gene Family and Their Expression Patterns under Drought Stress
摘要
Abstract
In the higher plants,SQUAMOSA promoter binding protein-box(SBP)is widely involved in plant growth,flowering regulation and programmed cell death.In this study,the sorghum SBP genes were i-dentified,and then their bioinformatics analysis was conducted and their expression under PEG-6000 simula-ted drought stress was analyzed using qRT-PCR.The results showed that 19 members of sorghum SBP family were identified.Except the 8th chromosome,the SBP genes were distributed on all the other 9 chromosomes.The SBP proteins were hydrophilic proteins,and most of them were located in nucleus at subcellular level.Similar to those of Oryza sativa,the sorghum SBP members also could be divided into three groups through phylogenetic analysis,containing 7,5 and 7 members respectively;sorghum SBPs had closer relationships with those of Oryza sativa and Zea mays.The prediction results of cis-acting elements indicated that sorghum SBPs might participate in abscisic acid and methyl jasmonate signaling pathways,and some members might be related to plant low temperature response and circadian rhythm regulation.qRT-PCR analysis results showed that the expression of sorghum SBP genes were tissue-specific,and 17 genes were induced to up-regulate ex-pression under simulated drought conditions and showed differentiated expression patterns with the extension of stress time.These results could provide theoretical bases for subsequent research on SBP genes function.关键词
高粱/SBP基因家族/生物信息学分析/基因表达/干旱胁迫Key words
Sorghum bicolor L./SBP gene family/Bioinformatics analysis/Gene expression/Drought stress分类
农业科技引用本文复制引用
姜昱雯,陈满静,赵应,任艳,周棱波,沈佳奇,邵明波..高粱SBP基因家族鉴定及干旱胁迫下的表达模式分析[J].山东农业科学,2024,56(3):1-10,10.基金项目
贵州省科技计划项目(黔科合基础-ZK[2023]一般169) （黔科合基础-ZK[2023]一般169）
贵州省基层农技推广项目(仁怀基地示范[2301]01号) （仁怀基地示范[2301]01号）
贵州省科技成果应用及产业计划项目(黔科中引地[2022]4045) （黔科中引地[2022]4045）
