中国食品添加剂2024，Vol.35Issue(3)：1-10,10.DOI:10.19804/j.issn1006-2513.2024.3.001
金银花多酚和多糖同步提取工艺优化及其抗氧化活性研究
Optimization of simultaneous extraction process of polyphenols and polysaccharides from Lonicera japonica Thunb.and their antioxidant activity
摘要
Abstract
Taking Lonicera japonica Thunb.(LJT)as raw material,the single-factor experiment of simultaneous extraction was carried out with six factors including ammonium sulphate concentration,ethanol content,solid-liquid ratio,ultrasonic time,extraction temperature and ultrasonic power.Based on the single-factor experiment,response surface method was used to optimize ultrasonic-assisted ethanol-ammonium sulphate aqueous two-phase extraction process for simultaneous extraction of polyphenols and polysaccharides from LJT.The extraction amounts of polyphenols and polysaccharides were selected as indicators.and the antioxidant activity in vitro of polyphenols and polysaccharides from LJT was investigated.The results showed that the optimum extraction conditions were as follows:ammonium sulphate concentration 0.2 mg/mL,ultrasonic time 30 min,ultrasonic temperature 59℃and solid-liquid ratio 1∶70(g/mL).Under the above conditions,the extraction amounts of polyphenols was 25.6 mg/g and the extraction amount of polysaccharides was 152.11 mg/g.In vitro antioxidant experiments showed that both polyphenols and polysaccharides from LJT had obvious scavenging ability to DPPH and ABTS free radicals,and strong reducing ability to Fe3+.Ultrasonic-assisted aqueous two-phase extraction could be used to extract polysaccharides and polyphenols from LJT simultaneously.The antioxidant activities of polysaccharides and polyphenols were significant and the antioxidant capacity of LJT polyphenols was stronger than VC in a certain range.关键词
金银花/双水相提取/同步提取/响应面/抗氧化活性Key words
Lonicera japonica Thunb./aqueous two-phase extraction/synchronous extraction/response surface/antioxidant activity分类
轻工纺织引用本文复制引用
梁金月,周洪鑫,张雨晴,王玲娜,李佳,赵东升..金银花多酚和多糖同步提取工艺优化及其抗氧化活性研究[J].中国食品添加剂,2024,35(3):1-10,10.基金项目
国家自然科学基金项目(81903750) （81903750）
山东省重点研发计划(2022TZXD0036) （2022TZXD0036）
山东中医药大学中药资源保护与质量评价青创团队支持项目 （）
山东中医药大学大学生创新创业训练计划项目(2023061). （2023061）
