春砂仁多糖对高脂饮食小鼠脂代谢和抗氧化作用的影响OACSTPCD

To explore the effect of Amomum villosum polysaccharide(AVP)on abnormal lipid metabolism and oxidative stress in mice induced by high-fat diet for 8 weeks.A total of 120 6-week-old healthy C57BL/6 male mice were randomly divided into control check group(CK),high-fat diet group(HFD),HFD+AVP low-dose group(HFD+L-AVP),HFD+AVP medium-dose group(HFD+M-AVP)and HFD+AVP high-dose group(HFD+H-AVP).The results showed that,in the group fed with AVP in the diet,the mice serum aspartate transaminase(AST),alanine aminotransferase(ALT),low-density lipoprotein(LDL-C)levels,triglyceride(TG)and total cholesterol(TC)were all significantly decreased.The high-density lipoprotein(HDL-C)in the serum of mice increased significantly.The activities of superoxide dismutase(SOD),peroxidase(CAT)and glutathione peroxidase(GSH-Px)in the liver and intestine of mice with high-fat diet were increased,and the level of malondialdehyde(MDA)was significantly reduced.AVP decreased mRNA expression level of cholesterol regulatory element binding protein 1(SREBP1)and fatty acid synthase(FAS)in high-fat diet mice,and mRNA expression level of triglyceride hydrolase(ATGL)in high-fat diet mice was increased.AVP significantly increased expression level of nuclear factor E2 related factor 2(Nrf2),heme oxygenase 1(HO-1)mRNA and protein in the intestine of high-fat diet mice and improved the intestinal antioxidant capacity.To sum up,Amomum villosum polysaccharide alleviated oxidative stress and abnormal lipid metabolism in mice caused by high-fat diet.