中国食品添加剂2024，Vol.35Issue(3)：246-253,8.DOI:10.19804/j.issn1006-2513.2024.3.030
固相萃取-高效液相色谱法检测食品中酸性大红GR
Detection of Brilliant Crocein in food by solid phase extraction-high performance liquid chromatography
摘要
Abstract
A method for determination of Brilliant Crocein in a variety of foods was developed.Extraction solvent,purification and chromatographic condition were optimized.The sample was extracted with 1%ammoniated methanol(v∶v),and purified and concentrated by a weak anionic solid phase extraction columns(PWAX).The separation of targeted compound was performed on a Titan C18 column using 10 mmol ammonium acetate-methanol as mobile phase and a diode-array detector.The results showed that Brilliant Crocein had a good linear relationship in the range of 0.040〜10.00 μg/mL and a good correlation coefficient(R2＞0.999).Limit of detection(LOD)was 0.025 mg/kg,and Limit of Quantitation(LOQ)was 0.1 mg/kg.When three levels of 0.10,0.20 and 1.00 mg/kg of Brilliant Crocein were added to different negative samples,the average recoveries were from 80.14%to 102.60%and relative standard deviations(RSDs)were 1.08%〜4.29%.The accuracy of the method was verified by comparing it with the method of supplementary inspection of food.This method was suitable for the detection of Brilliant Crocein in chili products,dried aquatic products and drinks.The research provides strong technical support for food safety and risk management control.关键词
非法染料/酸性大红GR/高效液相色谱法/多种食品Key words
illegal dyes/Brilliant Crocein GR/HPLC/a variety of foods分类
轻工纺织引用本文复制引用
武传香,魏莉莉,薛霞,卢兰香,丁一,高敏,王骏,刘艳明..固相萃取-高效液相色谱法检测食品中酸性大红GR[J].中国食品添加剂,2024,35(3):246-253,8.基金项目
国家食品安全关键技术研发重点专项(2017YEC1601600). （2017YEC1601600）
