安徽医科大学学报2024，Vol.59Issue(2)：212-218,7.DOI:10.19405/j.cnki.issn1000-1492.2024.02.005
大麻素2型受体在加速正畸牙移动中的作用
The role of CB2 in accelerating orthodontic tooth movement
摘要
Abstract
Objective To explore the effect of cannabinoid receptor 2(CB2)on orthodontic tooth movement(OTM)rate and periodontal tissue reconstruction of pressure area in mice.Methods Thirty CB2-/-male mice and thirty littermate control WT male mice were individually accepted the orthodontic appliance at their age of 6 weeks.The mice were respectively scarified at 3 days,7 days,14 days and 21 days after the operation.Then the tooth movement distance was examined through the stereomicroscope.Hematoxylin-eosin staining was performed to explore the biological responses of periodontium at the distal mesial root pressure area.Anti-tartrate acid phospha-tase staining was performed to calculate the number and distribution of osteoclasts at the distal mesial root pressure area,and MMP-9 was evaluated by immunohistochemistry to examine the number of MMP-9(+)monocytes and multinucleated cells in the same district as the TRAP staining.Results Compared with those WT mice at 3,7,14 and 21 days,OTM distance showed a gradual increased tendency according with experimental time over 21 days.The widths of periodontal ligament on the pressure side were markedly greater in CB2-/-mice than WT mice at 7,14 and 21 days(P＜0.000 1).The numbers of TRAP positive osteoclasts were significantly greater in CB2-/-mice than those in WT mice at 14 days of OTM(P＜0.001).MMP-9 immunohistochemical staining showed that the number of MMP-9(+)monocytes and multinucleated cells was more in CB2-/-mice than that in WT mice at 14 days of OTM(P＜0.05).Conclusion The absence of CB2 accelerates orthodontic tooth movement under or-thodontic force.The absence of CB2 reinforces bone resorption in orthodontic tooth movement compressive area dur-ing orthodontic tooth movement.关键词
大麻素2型受体/正畸牙移动/压力区/骨改建/破骨细胞Key words
cannabinoid receptor 2/orthodontic tooth movement/compressive area/bone remodelling/osteoclast分类
医药卫生引用本文复制引用
范登莹,翟浩嫣,刘慧娟,赵圆,李东娜,乔星,康文静,朱德超,刘春艳..大麻素2型受体在加速正畸牙移动中的作用[J].安徽医科大学学报,2024,59(2):212-218,7.基金项目
2021年政府资助临床医学优秀人才项目(编号:361029) （编号:361029）
2022年引进留学人员资助项目(编号:C20220353) （编号:C20220353）
