安徽医科大学学报2024，Vol.59Issue(2)：224-229,6.DOI:10.19405/j.cnki.issn1000-1492.2024.02.007
华蟾素通过调控M2型巨噬细胞极化抑制结直肠癌转移
Cinobufacini inhibits the metastasis of colorectal cancer by regulating polarization of M2 macrophages
摘要
Abstract
Objective To investigate the effect of cinobufacini on inhibiting colorectal cancer metastasis by regula-ting the polarization of M2 macrophages.Methods THP-1 was induced into M0 type macrophages.The condi-tioned medium of HCT116 cells was collected to stimulate M0 type macrophages.The polarization of M2 type mac-rophages was observed by flow cytometry,real-time quantitative PCR and ELISA experiments.The conditioned me-dium of M0 type macrophages and HCT116-Mφ cells was collected to stimulate HCT116 cells.The ability of migra-tion and invasion was observed by wound healing assay and Transwell assay.The effect of cinobufacini on the via-bility of HCT116 cells was detected by CCK-8 assay.The conditioned medium of HCT116 and HCT116+cinobufa-cini was collected to stimulate M0 type macrophages.The polarization of M2 type macrophages was observed by flow cytometry,real-time quantitative PCR and ELISA experiments.The conditioned media of HCT116-Mφ cells and(HCT116+cinobufacini)-Mφ cells were collected to stimulate HCT116 cells.The changes of migration and inva-sion ability were observed by wound healing assay and Transwell assay.Results After stimulation of M0 type mac-rophages in HCT116 cell conditioned medium,the morphology of M0 macrophages turned into fusiform cells,the proportion of CD11b+CD206+cells increased,and the expression of M2 macrophage markers IL-10 and TGF-β in-creased.The migration and invasion ability of HCT116 cells were significantly enhanced after stimulation in the conditioned medium of HCT1 16-Mφ cells.After the addition of cinobufacini,not only the polarization proportion of M2 macrophages decreased,but also the metastatic effect mediated by M2 macrophages was inhibited.Conclusion HCT116 cells can induce the polarization of M2 macrophages,while cinobufacini can inhibit the tumor metastasis mediated by M2 macrophages by inhibiting the polarization of M2 macrophages.关键词
华蟾素/结直肠癌/肿瘤相关巨噬细胞/转移Key words
cinobufacini/colorectal cancer/tumor-associated macrophage/metastasis分类
医药卫生引用本文复制引用
尚靖,王云,陈进宝,唐东豪,贾琳琳,李炜,于宏杰..华蟾素通过调控M2型巨噬细胞极化抑制结直肠癌转移[J].安徽医科大学学报,2024,59(2):224-229,6.基金项目
上海市启明星项目(扬帆专项)(编号:22YF1441400) （扬帆专项）
上海市普陀区临床特色专病建设项目(编号:2020tszb03) （编号:2020tszb03）
上海中医药大学附属普陀医院百人计划(领航计划)(编号:2022-RCLH-03) （领航计划）
成都中医药大学"杏林学者"学科人才科研提升计划(编号:YYZX2020121) （编号:YYZX2020121）
