草莓茎基腐病病原菌鉴定、致病力分析及防治药剂筛选OA北大核心CSTPCD

Zhejiang stands as the primary cultivation region for strawberries in China.To clarify the pathogen causing strawberry stem rot in Jiande city and screen out effective control agents,samples exhibiting typical symptoms of strawberry stem rot were collected from the strawberry base in Jiande,Zhejiang Province,the pathogen was isolated and identified,and the biological characteristics,host range of the pathogen,and effective bactericides were studied.The results showed that a yellow,round,sticky,transparent,and pungent colony was isolated from the boundary between the diseased and healthy parts of the stem base,and it was identified as Pantoea ananatis according to the morphological,molecular biology,and biological characteristics.The pathogen prefers growing in the neutral to alkaline environment,exhibiting optimal growth at pH 8,28℃,and salt concentration of 1%to 5%.Pathogenicity tests confirmed that the P.ananatis isolated from strawberry was able to infect tomato,pepper,watermelon,and rice,but could not infect Chinese cabbage,broccoli,and beans,which showed notable pathogenicity disparities compared with the P.ananatis isolated from rice.The laboratory tests revealed that four of the 20 tested agents had bacteriostatic effects on P.ananatis.Among which 50%tetramethylthiuram disulfide wettable powder exhibited the highest inhibitory effects with an EC50 of 20.805 7 mg·L-1,which could be used as the primary selection agent for controlling the strawberry stem rot.This research laid the foundation for the effective control of strawberry stem rot and furnished valuable insights for unraveling the pathogenic mechanisms associated with P.ananatis.