S100A9基因敲除对姥鲛烷诱导小鼠狼疮肾炎的影响OA北大核心CSTPCD

Objective:To explore the effects of S100A9 knockout on mice with lupus nephritis(LN)induced by pristane,and clarify specific roles of S100A9 in LN.Methods:Ten female wild-type(WT)C57BL/6(B6)mice and ten S100A9-/-B6 mice(6-8 weeks old)were used.Five WT B6 mice and five S100A9-/-B6 mice were intraperitoneally injected with 0.5 mL pristane,respectively,serving as experimental groups.Other five WT B6 mice and five S100A9-/-B6 mice were intraperitoneally injected with 0.5 mL normal saline,respectively,serving as control groups.The mice were sacrificed at six months after injection.The expression of anti-double-stranded DNA(ds-DNA)antibody was measured by ELISA.The levels of serum creatinine,serum urea nitrogen and urine protein were detected.Renal tissues were collected for HE staining to evaluate renal pathology.Results:There were no significant differences between WT and S100A9-/-B6 mice on mouse body-weight,spleen weight,kidney-structure,and serum creatitine levels etc.Compared with control B6 mice,pristane treated B6 mice showed increased weight and length of spleen as well as increased levels of serum creatinine,urea nitrogen,proteinuria,anti-ds-DNA antibody and IgG,and the lupus-like changes in the kidney with increased glomerular volume,edema or renal tuble epithelium,and stenosis of lumen.Similar results were observed in pristane-treated S100A9-/-B6 mice,compared with control S100A9-/-B6 mice.However,compared with pristane-treated WT B6 mice,the above symptoms of LN and indexes of serum and urine were mild in pristane-treated S100A9-/-B6 mice.Conclusion:Pristane induces systemic lupus erythematosus in B6 mice and S100A9-/-B6 mice.However,the degree of lesions in mice with S100A9 gene knockout is reduced,suggesting that this gene may have a promoting effect on the pathogenesis of lupus in mice.