热带作物学报2024，Vol.45Issue(3)：450-458,9.DOI:10.3969/j.issn.1000-2561.2024.03.002
木薯MeHsfB3b转录因子与MeFKBP20蛋白互作关系验证
Verification of the Interaction Between Cassava MeHsfB3b Tran-scription Factor and MeFKBP20 Protein
摘要
Abstract
The heat shock transcription factor MeHsfB3b is important in regulating the resistance to cassava bacterial blight.In the previous study,the candidate interaction protein MeFKBP20 of MeHsfB3b was obtained by the yeast two-hybrid technology,which belongs to the FKBP-type peptide prolyl cis-trans isomerase gene family.In this study,the full-length MeFKBP20 gene was cloned to be 561 bp,encoding 186 amino acids,with a molecular weight of 19.99 kDa and a FKPB_C domain.The expression pattern analysis showed that MeFKBP20 was highly expressed in mature leaves and roots of cassava,and the expression level of young leaves and petioles was low.It could quickly respond to the in-duction of pathogen XpmCHN11 and maintain a high expression abundance,suggesting that it is involved in response to pathogen infection.The interaction between MeHsfB3b protein and MeFKBP20 protein through 201-241 aa region was proved by the yeast two-hybrid point-to-point and bimolecular fluorescence complementation experiments.The results of this study are helpful to further analyze the mechanism of MeHsfB3b gene regulating cassava resistance to cassava bacterial blight.关键词
热激转录因子/FKBP型肽脯氨酰顺反异构酶/酵母双杂交/双分子荧光互补Key words
heat shock transcription factor/FKBP-type peptide prolyl cis-trans isomerase/yeast two-hybrid/bimolecular fluorescence complementation分类
农业科技引用本文复制引用
王超群,李琳琳,陈银华,张肖飞,姚远,耿梦婷..木薯MeHsfB3b转录因子与MeFKBP20蛋白互作关系验证[J].热带作物学报,2024,45(3):450-458,9.基金项目
海南省自然科学基金项目(No.320RC492) （No.320RC492）
海南省研究生创新科研课题(No.Qhys2021-178). （No.Qhys2021-178）
