食品科学2024，Vol.45Issue(6)：55-63,9.DOI:10.7506/spkx1002-6630-20230613-098
基于RBL-2H3细胞脱颗粒抑制作用的体外抗过敏活性益生菌的筛选及鉴定
Screening and Identification of Probiotics with in Vitro Anti-Allergic Activity Based on the Degranulation Inhibition of RBL-2H3 Cells
摘要
Abstract
To investigate the anti-allergic activity of lactic acid bacteria,lactic acid bacterial strains were obtained from fermented vegetables by the traditional isolation technique and screened for in vitro allergic activity by the degranulation inhibition assay against RBL-2H3 cells.The results showed that all four isolates exhibited good probiotic properties and safety and had a strong inhibitory effect on β-hexosaminidase released from RBL-2H3 cells after degranulation.Moreover,they showed significant down-regulation effects on the release of histamine,tumor necrosis factor-α and interleukin-4 from RBL-2H3 cell after degranulation,suggesting their potential as anti-allergic probiotics.The four strains were identified by 16S rDNA sequencing as two strains of Lactobacillus plantarum,one strain of L.fermentum and one strain of L.paracasei.关键词
乳酸菌/RBL-2H3细胞/益生特性/脱颗粒/抗过敏活性Key words
lactic acid bacteria/RBL-2H3 cells/probiotic properties/degranulation/anti-allergic activity分类
轻工业引用本文复制引用
马丁,秦双霞,郝慧超,邓放明,赵玲艳..基于RBL-2H3细胞脱颗粒抑制作用的体外抗过敏活性益生菌的筛选及鉴定[J].食品科学,2024,45(6):55-63,9.基金项目
国家现代农业产业技术体系建设专项(CARS-24-E-02) （CARS-24-E-02）
湖南农业大学"双一流"建设项目(SYL20182006) （SYL20182006）
植物源益生菌菌种资源库建设及开发应用研究项目(AU-YJY-B-LX-20-014) （AU-YJY-B-LX-20-014）
湖南省自然科学基金面上项目(2021JJ30338) （2021JJ30338）
发酵辣椒绿色精深加工工程技术研究中心建设项目(2023ZYQ044) （2023ZYQ044）
