食品科学2024，Vol.45Issue(6)：97-104,8.DOI:10.7506/spkx1002-6630-20221220-203
两种生育酚酯衍生物对D-半乳糖致衰老小鼠的抗衰老作用
Anti-Aging Effects of Two Tocopherol Ester Derivatives on D-Galactose-Induced Aging in Mice
摘要
Abstract
In order to explore the anti-aging effects of two tocopherol ester derivatives,the in vitro antioxidant capacity of D-α-tocopherol acetate and DL-α-tocopherol acetate was determined.A mouse model of D-galactose-induced was created and intervened with each of the tocopherol ester derivatives for 42 days.Changes in aging related indicators were analyzed.The results showed that the hydroxyl radical scavenging capacity,and the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)cation radical scavenging capacity,Fe2+ chelating capacity,and total reducing power of the two tocopherol ester derivatives were better than those of vitamin C in the concentration range of 0.1 to 0.3 mg/mL.Compared with the aging model group,after intervention with tocopherol ester derivatives,the content of malondialdehyde(MDA)in serum decreased significantly(P<0.05,P<0.01,P<0.001,and P<0.000 1),and glutathione peroxidase(GSH-Px)activity and total antioxidant capacity(T-AOC)increased significantly(P<0.05,P<0.01,P<0.001,and P<0.000 1).The serum levels of inflammatory cytokines such as interleukin-6(IL-6),interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α),and liver function indexes including aspartate aminotransferase(AST)and alanine aminotransferase(ALT)decreased significantly(P<0.05,P<0.01,P<0.001,and P<0.001).Moreover,the relative mRNA and protein expression levels of nuclear factor-E2-related factors(Nrf2),quinone oxidoreductase(NQO1)and heme oxygenase 1(HO-1)in the mouse liver were enhanced.DL-α-tocopherol acetate increased the relative mRNA expression of Nrf2,NQO1 and HO-1 by 514.08%,461.78%and 515.32%,respectively,and their relative protein expression by 620.00%,988.89%and 1 200.00%,respectively(P<0.000 1).To sum up,the two tocopherol ester derivatives have anti-aging effects on D-galactose-induced aging in mice,DL-α-tocopherol acetate being more effective than D-α-tocopherol acetate,and their anti-aging effects may be related to the regulation of the Nrf2 signaling pathway.关键词
生育酚酯衍生物/D-半乳糖/抗氧化/抗衰老/核因子-E2-相关因子Key words
tocopherol ester derivatives/D-galactose/antioxidant/anti-aging/nuclear factor E2 related factor 2分类
医药卫生引用本文复制引用
陈炜莉,贝翎,杨扬,王璧莹,李家旭,阚绪甜,李文治,杜冰..两种生育酚酯衍生物对D-半乳糖致衰老小鼠的抗衰老作用[J].食品科学,2024,45(6):97-104,8.基金项目
财政部和农业农村部:国家现代农业产业技术体系建设专项(CARS-21) （CARS-21）
