食品科学2024，Vol.45Issue(6)：271-276,6.DOI:10.7506/spkx1002-6630-20230509-079
基于衍生化3-氨基-2-恶唑烷酮的夹心酶联免疫分析
A Sandwich Immunosorbent Assay for 3-Amino-2-Oxazolidinone Following Derivatization
摘要
Abstract
In the present study,a sandwich enzyme-linked immunosorbent assay(ELISA)for 3-amino-2-oxazolidnone(AOZ)was developed.A novel derivatizing agent for AOZ was synthesized by linking 2-nitro-4-carboxybenzaldehyde and biotin through 1,6-hexanediol.During the sample pretreatment,the agent was added for AOZ derivatization with an efficiency of 89%.The monoclonal antibody(McAb)against AOZ was coated on the microplate and horseradish peroxidase-labeled avidin or anti-biotin antibody was used as a secondary conjugate in the ELISA method.From a practical perspective,the limit distances between the two epitopes in the double-antibody sandwich and antibody-avidin sandwich modes were 12 and 13 Å,respectively,and the ideal distances were 16 and 17 Å,respectively.The detection limits of the double-antibody sandwich and antibody-avidin sandwich modes were 1.8 and 0.8 pg/mL for AOZ,respectively.Compared with competitive ELISA,a 25-fold improvement in the sensitivity of the developed ELISA method was achieved.The average recoveries and average relative standard deviations(RSDs)were 73%-85%and 9.0%,respectively.关键词
3-氨基-2-恶唑烷酮/夹心酶联免疫分析/表位间隔距离/辣根过氧化物酶标记亲和素Key words
3-amino-2-oxazolidinone/sandwich immunoassay/epitope spacing distance/horseradish peroxidase-labeled avidin分类
医药卫生引用本文复制引用
张世伟,吴会玲,周迎春,王炳志,杨星星,杜业刚,汤璐,冯荣虎,郭继平..基于衍生化3-氨基-2-恶唑烷酮的夹心酶联免疫分析[J].食品科学,2024,45(6):271-276,6.基金项目
深圳市科技计划项目(KCXFZ20230731092859013 （）
JCYJ20210324141804012 （）
JCYJ20210324141801003) （）
