食品科学2024，Vol.45Issue(5)：225-232,8.DOI:10.7506/spkx1002-6630-20230608-068
原料乳冷藏过程中差异表达蛋白的表征与比较分析
Characterization and Analysis of Differentially Expressed Proteins in Raw Milk during Cold Storage
摘要
Abstract
Using data independent acquisition(DIA)proteomics,the composition and biological functions of differentially expressed proteins in raw milk during cold storage at 4℃for up to 6 days were investigated.A total of 902 proteins were detected.Among them,70 and 71 differentially expressed proteins were selected during the early(day 0 vs day 3)and late(day 3 vs day 6)stages of refrigeration,respectively.The differentially expressed proteins played an important role in the response to nerve growth factors and the regulation of cells and tissues at the early stage of refrigeration,and were mainly involved in the biological processes of cells.At the late stage of refrigeration,the differentially expressed proteins could regulate the metabolic processes of carbohydrate and RNA,and mainly participated in the sugar metabolic pathways.Protein-protein interaction(PPI)network analysis indicated that cell division control protein 42 homolog(CDC42)was the hub node protein for both refrigeration periods,which was closely related to phagocytosis.These findings revealed the compositional changes and functional diversity of functions in raw milk proteins during refrigeration,which will provide a theoretical basis for the cold storage of raw milk and be of great significance for the quality control of raw milk.关键词
原料乳/冷藏/蛋白组学/差异表达蛋白/生物信息学Key words
raw milk/refrigeration/proteomics/differentially expressed proteins/bioinformatics分类
轻工纺织引用本文复制引用
庄姣,剧柠,朱晓雪,李亚凤,丁雨红,高岩,陈彦辉,齐瑾,杨秉坤..原料乳冷藏过程中差异表达蛋白的表征与比较分析[J].食品科学,2024,45(5):225-232,8.基金项目
国家自然科学基金地区科学基金项目(32160593) （32160593）
