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首页|期刊导航|食品科学|羧甲基壳聚糖诱导罗伦隐球酵母对葡萄柚果实细胞壁代谢的影响

羧甲基壳聚糖诱导罗伦隐球酵母对葡萄柚果实细胞壁代谢的影响OA北大核心CSTPCD

Effect of Carboxymethyl Chitosan on Cell Wall Metabolism of Grapefruits Induced by Cryptococcus laurentii

中文摘要英文摘要

以'里约红'葡萄柚为试材,对羧甲基壳聚糖(carboxymethyl chitosan,CMCS)、罗伦隐球酵母(Cryptococcus laurentii)以及CMCS-C.laurentii处理葡萄柚果实采后的硬度进行测定,结果显示:与对照组(无菌水处理)相比,CMCS-C.laurentii处理显著抑制了葡萄柚果实在整个贮藏期间内的果实硬度下降(P<0.05).在此基础上,测定贮藏期间(75 d)葡萄柚果实质量损失率、细胞壁多糖物质含量和细胞壁水解酶活力并观察果实细胞壁微观结构.结果表明:CMCS-C.laurentii处理能有效维持果实内共价结合型果胶、紧密型半纤维素的含量,降低果实中水溶性果胶、离子型果胶以及松散型半纤维素的含量;并且相较于对照组,CMCS-C.laurentii处理组果实细胞壁水解酶(果胶甲酯酶、多聚半乳糖醛酸酶、纤维素酶、α-L-阿拉伯呋喃糖苷酶和β-葡萄糖苷酶)活性均受显著抑制;贮藏中后期,CMCS-C.laurentii处理的果实细胞壁结构更加稳定,胞内结构相对完整.综上所述,CMCS-C.laurentii处理能减缓采后葡萄柚果实细胞内部细胞器及细胞壁的降解,较好地维持细胞完整性,延缓果实软化.

The hardness of'Rio Red'grapefruits treated postharvest with carboxymethyl chitosan(CMCS),Cryptococcus laurentii or C.laurentii induced by CMCS was measured during storage.The results showed that compared with the control group(treatment with sterile water),CMCS-C.laurentii treatment significantly maintained the firmness of grapefruits during storage(P<0.05).Moreover,fruit mass loss percentage and the polysaccharide content and polysaccharide hydrolase activity of the cell wall were measured and cell wall microstructure was observed during storage for 75 days.The results indicated that CMCS-C.laurentii treatment was effective in maintaining the contents of sodium carbonate soluble pectin(SCSP)and 24%KOH-soluble fraction(24KSF)and reducing the contents of water-soluble pectin(WSP),ionic-soluble pectin(ISP)and 4%KOH-soluble fraction(4KSF)in the fruit.Compared with the control group,the activities of the cell wall-degrading enzymes pectin methylesterase(PE),polygalacturonase(PG),cellulase(CX),α-L-arabinofuranosidase(α-L-Af)and β-galactosidase(β-Gal)were significantly inhibited by CMCS-C.laurentii treatment.In the middle and later stages of storage,the cell wall structure of the fruit treated with CMCS-C.laurentii was more stable and the intracellular structure was relatively intact.In conclusion,CMCS-C.laurentii treatment can slow down the degradation of organelles and cell walls in postharvest grapefruits,maintain cell integrity better and delay fruit softening.

杜嵘宇;黄二宾;吴化雨;王芳;邓佳

西南林业大学林学院,云南 昆明 650224西南林业大学林学院,云南 昆明 650224||西南地区生物多样性保育国家林业局重点实验室,云南 昆明 650224西南林业大学林学院,云南 昆明 650224||西南山地森林资源保育与利用教育部重点实验室,云南 昆明 650224

轻工业

果实软化葡萄柚羧甲基壳聚糖罗伦隐球酵母细胞壁代谢

fruit softeninggrapefruitscarboxymethyl chitosanCryptococcus laurentiicell wall metabolism

《食品科学》 2024 (005)

243-249 / 7

国家自然科学基金地区科学基金项目(31960326;32160394);云南省农业联合专项(202101BD070001-065);云南省"高层次人才培养支持计划"项目(YNWR-QNBJ-2020-205);云南省"两类"人才培养项目(202105AC160045)

10.7506/spkx1002-6630-20230220-185

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