食品科学2024，Vol.45Issue(5)：243-249,7.DOI:10.7506/spkx1002-6630-20230220-185
羧甲基壳聚糖诱导罗伦隐球酵母对葡萄柚果实细胞壁代谢的影响
Effect of Carboxymethyl Chitosan on Cell Wall Metabolism of Grapefruits Induced by Cryptococcus laurentii
摘要
Abstract
The hardness of'Rio Red'grapefruits treated postharvest with carboxymethyl chitosan(CMCS),Cryptococcus laurentii or C.laurentii induced by CMCS was measured during storage.The results showed that compared with the control group(treatment with sterile water),CMCS-C.laurentii treatment significantly maintained the firmness of grapefruits during storage(P<0.05).Moreover,fruit mass loss percentage and the polysaccharide content and polysaccharide hydrolase activity of the cell wall were measured and cell wall microstructure was observed during storage for 75 days.The results indicated that CMCS-C.laurentii treatment was effective in maintaining the contents of sodium carbonate soluble pectin(SCSP)and 24%KOH-soluble fraction(24KSF)and reducing the contents of water-soluble pectin(WSP),ionic-soluble pectin(ISP)and 4%KOH-soluble fraction(4KSF)in the fruit.Compared with the control group,the activities of the cell wall-degrading enzymes pectin methylesterase(PE),polygalacturonase(PG),cellulase(CX),α-L-arabinofuranosidase(α-L-Af)and β-galactosidase(β-Gal)were significantly inhibited by CMCS-C.laurentii treatment.In the middle and later stages of storage,the cell wall structure of the fruit treated with CMCS-C.laurentii was more stable and the intracellular structure was relatively intact.In conclusion,CMCS-C.laurentii treatment can slow down the degradation of organelles and cell walls in postharvest grapefruits,maintain cell integrity better and delay fruit softening.关键词
果实软化/葡萄柚/羧甲基壳聚糖/罗伦隐球酵母/细胞壁代谢Key words
fruit softening/grapefruits/carboxymethyl chitosan/Cryptococcus laurentii/cell wall metabolism分类
轻工纺织引用本文复制引用
杜嵘宇,黄二宾,吴化雨,王芳,邓佳..羧甲基壳聚糖诱导罗伦隐球酵母对葡萄柚果实细胞壁代谢的影响[J].食品科学,2024,45(5):243-249,7.基金项目
国家自然科学基金地区科学基金项目(31960326 （）
32160394) （）
云南省农业联合专项(202101BD070001-065) （202101BD070001-065）
云南省"高层次人才培养支持计划"项目(YNWR-QNBJ-2020-205) （YNWR-QNBJ-2020-205）
云南省"两类"人才培养项目(202105AC160045) （202105AC160045）
