羧甲基壳聚糖诱导罗伦隐球酵母对葡萄柚果实细胞壁代谢的影响OA北大核心CSTPCD

The hardness of'Rio Red'grapefruits treated postharvest with carboxymethyl chitosan(CMCS),Cryptococcus laurentii or C.laurentii induced by CMCS was measured during storage.The results showed that compared with the control group(treatment with sterile water),CMCS-C.laurentii treatment significantly maintained the firmness of grapefruits during storage(P<0.05).Moreover,fruit mass loss percentage and the polysaccharide content and polysaccharide hydrolase activity of the cell wall were measured and cell wall microstructure was observed during storage for 75 days.The results indicated that CMCS-C.laurentii treatment was effective in maintaining the contents of sodium carbonate soluble pectin(SCSP)and 24%KOH-soluble fraction(24KSF)and reducing the contents of water-soluble pectin(WSP),ionic-soluble pectin(ISP)and 4%KOH-soluble fraction(4KSF)in the fruit.Compared with the control group,the activities of the cell wall-degrading enzymes pectin methylesterase(PE),polygalacturonase(PG),cellulase(CX),α-L-arabinofuranosidase(α-L-Af)and β-galactosidase(β-Gal)were significantly inhibited by CMCS-C.laurentii treatment.In the middle and later stages of storage,the cell wall structure of the fruit treated with CMCS-C.laurentii was more stable and the intracellular structure was relatively intact.In conclusion,CMCS-C.laurentii treatment can slow down the degradation of organelles and cell walls in postharvest grapefruits,maintain cell integrity better and delay fruit softening.