食品科学2024，Vol.45Issue(5)：265-274,10.DOI:10.7506/spkx1002-6630-20230822-160
基于石墨烯泡沫的电增强固相微萃取-高效液相色谱法测定鱼肉中3种磺胺类的残留
Determination of the Residues of Three Sulfonamides in Fish by Graphene Foam-Based Electro-Enhanced Solid-Phase Microextraction Coupled with High Performance Liquid Chromatography
摘要
Abstract
A rapid and sensitive method for the determination of sulfonamide(SA)residues in fish was developed using electro-enhanced solid-phase microextraction(EE-SPME)based on nitrogen-doped three-dimensional graphene foam(NGF)functionalized monolithic column(monolith@NGF,M@NGF)combined with high performance liquid chromatography(HPLC).To improve the enrichment efficiency of SA residues in fish,porous M@NGF was prepared on the surface of stainless-steel wires by in situ polymerization using ionic liquids as the functional monomer and NGF as the conductivity enhancer.The EE-SPME method was applied for the extraction of sulfathiazole(ST),sulfamethazine(SM2),and sulfadimethoxypyrimidine(SMM)from fish.Adsorption time,agitation speed,adsorption voltage,ionic strength,the pH of sample solution,desorption time,desorption voltage,and the composition of desorption solution were optimized.The results showed that the M@NGF-based EE-SPME method enriched ST,SM2 and SMM by 74,58 and 64 folds,respectively,and shortened the adsorption equilibrium time to 35 min.In the linear range of 5-5 000 μg/kg,the limits of detection(LODs)of this method were 1.78,3.16 and 1.84 μg/kg for ST,SM2 and SMM,respectively,and the limits of quantification(LOQs)were 5 μg/kg for all analytes.Coefficients of determination(R2)for linear regression analysis of this method were all greater than 0.999 0,and the recoveries from spiked samples ranged from 79.2%to 110.1%,with relative standard deviations(RSDs)ranging from 1.4%to 9.8%(n = 5).This method solved the problems of low extraction efficiency and serious matrix interference of SA residues in fish,and allowed the rapid,sensitive detection of SA residues in fish.关键词
石墨烯泡沫/电增强固相微萃取/高效液相色谱法/磺胺类残留/鱼肉Key words
graphene foam/electro-enhanced solid-phase microextraction/high performance liquid chromatography/sulfonamide residues/fish分类
轻工纺织引用本文复制引用
孙瑞雪,方禹文,陈冬妍,陈全胜,陈晓梅..基于石墨烯泡沫的电增强固相微萃取-高效液相色谱法测定鱼肉中3种磺胺类的残留[J].食品科学,2024,45(5):265-274,10.基金项目
国家自然科学基金面上项目(32072299) （32072299）
