生物加工过程2024，Vol.22Issue(2)：139-146,8.DOI:10.3969/j.issn.1672-3678.2024.02.003
嘌呤核苷磷酸化酶/嘧啶核苷磷酸化酶共表达及固定化催化合成阿糖核苷
Co-expression of purine nucleoside phosphorylase and uridine phosphorylase in Escherichia coli for the synthesis of arabinoside
摘要
Abstract
Biocatalysis has demonstrated great potential in the synthesis of nucleoside analogues due to its mild reaction conditions,environmental friendliness and sustainability.As frequently used enzymes in biocatalytic approaches,nucleoside phosphorylases attracted great attention in recent years.Herein,the co-expression of purine and pyrimidine nucleoside phosphorylases was achieved in an engineered Escherichia coli(E.coli),which could overcome the limitation and reversibility of substrates in the transformation catalyzed by single nucleoside phosphorylase.The engineered E.coli strain,which was constructed through overexpression of both endogenous genes deoD and udp,was used for biosynthesis of different nucleoside analogues.As a result,the co-expression engineered strain could effectively catalyze the conversion of different arabinosides,with excellent preference for purine over pyrimidine.In addition,the immobilized double enzymes could further improve the substrate conversion rate and prolong the service life of the enzyme,notably with the cumulative effective catalytic time up to 684 h.Our research provided valuable information for the biosynthesis of nucleoside analogues.关键词
嘌呤核苷磷酸化酶/嘧啶核苷磷酸化酶/阿糖核苷/固定化酶/生物催化Key words
purine nucleoside phosphorylases/uridine phosphorylase/arabinoside/immobilized enzyme/biocatalysis分类
生物科学引用本文复制引用
刘玉雪,张震,胡晓静,王振宇,刘国生..嘌呤核苷磷酸化酶/嘧啶核苷磷酸化酶共表达及固定化催化合成阿糖核苷[J].生物加工过程,2024,22(2):139-146,8.基金项目
河南省高等学校重点科研计划(20A180033) （20A180033）
河南省自然科学基金(222300420201) （222300420201）
