生物加工过程2024，Vol.22Issue(2)：196-203,8.DOI:10.3969/j.issn.1672-3678.2024.02.010
恙虫病东方体混合重组抗原应用于胶体金免疫层析试纸条的制备及效价检测
Establishment of colloidal gold immunochromatographic assay to detect the antibody against Orientia tsutsugamushi
摘要
Abstract
In recent years,colloidal gold immunochromatography strips were widely utilized in disease diagnosis,due to the convenience for carrying,simple operation,as well as high sensitivity and specificity.Herein,we developed a convenient approach for the rapid detection of Scrub typhus,using colloidal gold immunochromatographic strip to monitor the total antibody against Orientia tsutsugamushi.The double-antigen sandwich method was used in this case to spray the 56 kDa mixed recombinant protein of Orientia tsutsugamushi with gold onto the detection line,and the 56 kDa mixed recombinant protein and rabbit polyclonal antibody against Orientia tsutsugamushi onto the quality control line,respectively.As a result,our colloidal gold assay showed the detection limit of 61 ng/mL,and demonstrated no cross reactions with the positive sera of malaria,typhoid,schistosomiasis,haemorrhagic fever,spotted fever,etc.Moreover,the test strips proved to be stable at 4 ℃ for 6 months,and this method could provide excellent specificity of 90％(54/60)and the detection sensitivity of 91.96％(103/112).Therefore,our colloidal gold immunochromatography strip can be applied to detect total antibody against Orientia tsutsugamushi in human serum.关键词
恙虫病东方体/胶体金/混合重组蛋白/快速检测试纸条Key words
Orientia tsutsugamushi/colloidal gold/mixed recombinant protein/rapid test strip分类
医药卫生引用本文复制引用
赵芷若,周东明,操敏,陶清源,蔡旭燊,燕书豪,高豹,吕恒,谭伟龙..恙虫病东方体混合重组抗原应用于胶体金免疫层析试纸条的制备及效价检测[J].生物加工过程,2024,22(2):196-203,8.基金项目
国家自然科学基金面上项目(81472932) （81472932）
江苏省自然科学基金(BK20170998) （BK20170998）
国家科技重大专项子课题(2018ZX10713003、2017ZX10303401) （2018ZX10713003、2017ZX10303401）
军队后勤重大项目(AWS16J020) （AWS16J020）
